Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence.
Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence.
复制标题
通过免疫荧光检测禽类和哺乳动物致癌 RNA 病毒（癌病毒）。
作者：
J. Hilgers;R. Nowinski;G. Geering;W. Hardy
采用间接免疫荧光法和免疫荧光吸收法测定了禽、鼠、仓鼠和猫感染冠状病毒的细胞中病毒抗原的存在和细胞内沉积。在所有情况下，病毒抗原都局限于细胞质；未观察到核荧光。免疫荧光法和免疫荧光吸收法对小鼠肿瘤的筛选表明：(a)小鼠白血病病毒与小鼠乳腺肿瘤病毒（MTV）在血清学上不相关；(b)小鼠白血病病毒在小鼠群体中广泛存在，并发生在白血病高发病率和低发病率菌株小鼠的各种肿瘤和白血病中；(c) MTV仅限于几种类型的肿瘤组织，仅发生在乳腺肿瘤发生率高的小鼠株中。利用免疫荧光吸收技术，可以在高发株小鼠的脾脏中检测到MTV和小鼠白血病病毒。高乳腺肿瘤毒株（或高白血病毒株）与低发病率毒株杂交小鼠脾脏中病毒抗原的含量介于亲本毒株之间。小鼠与鸡、仓鼠和猫的冠状病毒的血清学比较显示：(A)禽类和哺乳动物的冠状病毒在血清学上彼此无关；(b)来自鸡、小鼠、仓鼠和猫的冠状病毒含有血清学上可区分的物种特异性病毒抗原（然而，单一物种内的白血病和肉瘤病毒在抗原性上无法区分）；(c)哺乳动物白血病肉瘤病毒具有至少一种共同抗原；(d)小鼠MTV在血清学上与所有其他冠状病毒无关。
Summary The indirect immunofluorescence and immunofluorescence absorption tests were used to determine the presence and intracellular deposition of viral antigens in cells infected with avian, murine, hamster, and feline oncornaviruses. In all instances, viral antigens were restricted to the cytoplasm; nuclear fluorescence was not observed. Screening of murine neoplasms by immunofluorescence and immunofluorescence absorption showed ( a ) that murine leukemia virus and murine mammary tumor virus (MTV) were serologically unrelated; ( b ) that murine leukemia virus was widespread throughout the mouse population and occurred in a variety of tumors and leukemias of mice from both high- and low-leukemia-incidence strains; and ( c ) that MTV was restricted to a few types of neoplastic tissues, which occurred only in mice of high-mammary-tumor-incidence strains. With the immunofluorescence absorption technique, MTV, as well as murine leukemia virus, could be detected in the spleen of individual mice from high-incidence strains. The amount of viral antigen in the spleen of mice from crosses of high-mammary-tumor strains (or high-leukemia strains) with mice of low-incidence strains was intermediate between that of the parental strains. A serological comparison of murine oncornaviruses with those of the chicken, hamster, and cat revealed that ( a ) the avian and mammalian oncornaviruses are serologically unrelated to each other; ( b ) oncornaviruses from the chicken, mouse, hamster, and cat contain species-specific viral antigens that are serologically distinguishable (however, the leukemia and sarcoma viruses within a single species are antigenically indistinguishable); ( c ) the mammalian leukemia-sarcoma viruses share at least one common antigen; and ( d ) the mouse MTV is serologically unrelated to all other oncornaviruses.