Tissue and cell specificity of immobilin biosynthesis.
Tissue and cell specificity of immobilin biosynthesis.
复制标题
固定蛋白生物合成的组织和细胞特异性。
DOI：
10.1095/biolreprod39.4.901
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Ruiz-Bravo,N
中科院分区：
文献类型：
--
作者：
Ruiz-Bravo,N
直到最近，对精子运动的启动机制仍知之甚少。Immobilin是一种新的高分子量粘蛋白糖蛋白，发现在尾部附睾的大鼠，在浓度相当于那些发现在原生尾部附睾液，可逆地抑制精子活力。本研究从大鼠附睾尾液中纯化出immobilin，并用于制备兔抗immobilin多克隆抗体。该抗体的特点是免疫印迹，免疫荧光法用于本地化immobilin在石蜡切片的成年雄性大鼠的生殖系统的组成部分。Immobilin在输出管中检测不到，并且首先在附睾头的初始段的一些上皮细胞的顶端部分中检测到。Immobilin仅在附睾头细胞内可检测到。在附睾体和尾部，immobilin仅在小管腔中可检测到。免疫沉淀的immobilin放射性标记在体外证实，immobilin的生物合成在成年大鼠仅限于附睾头。附睾头中的主细胞合成固定素并将其分泌到小管腔中，以便与精子一起进入尾部。
The mechanisms for the initiation of sperm motility have been poorly understood until recently. Immobilin is a novel mucin glycoprotein of high molecular weight found in the cauda epididymis of the rat that, at concentrations equivalent to those found in native cauda epididymal fluid, reversibly inhibits sperm motility. In this study, immobilin was purified from rat cauda epididymal fluid to apparent homogeneity and used to generate polyclonal antibody in rabbits. The antibody was characterized by immunoblotting, and immunofluorescence was used to localize immobilin in paraffin sections of components of the reproductive system of adult male rats. Immobilin was not detectable in the efferent duct and was first detectable in the apical portion of some epithelial cells of the initial segment of the caput epididymis. Immobilin was detectable intracellularly only in cells of the caput epididymis. In the corpus and cauda epididymis immobilin was detectable only in the lumen of the tubules. Immunoprecipitation of immobilin radiolabeled in vitro confirmed that immobilin biosynthesis in the adult rat is restricted to the caput epididymis. Principal cells in the caput epididymis synthesize immobilin and secrete it into the lumen of the tubules to travel with the sperm into the cauda.