Monosialyl-Gb5 organized with cSrc and FAK in GEM of human breast carcinoma MCF-7 cells defines their invasive properties.
Monosialyl-Gb5 organized with cSrc and FAK in GEM of human breast carcinoma MCF-7 cells defines their invasive properties.
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单唾液酸-Gb5 在人乳腺癌 MCF-7 细胞的 GEM 中与 cSrc 和 FAK 组织在一起，定义了它们的侵袭特性。
DOI：
10.1016/s0014-5793(02)03484-1
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发表时间：
2002
期刊：
影响因子：
3.5
通讯作者：
Hakomori,Senitiroh
中科院分区：
文献类型：
--
作者：
Steelant,WimF;Kawakami,Yasushi;Ito,Akihiro;Handa,Kazuko;Bruyneel,ErikA;Mareel,Marc;Hakomori,Senitiroh
两种人乳腺癌细胞变体MCF-7/AZ和MCF-7/6在其富含鞘糖脂的微结构域（GEM）中显示出相同的组成，包括globo系列结构Gb 3、Gb 4、Gb 5、单唾液酸-Gb 5、GM 2和cSrc以及FAK。两种变体均无创进入胶原凝胶层，并且在伤口迁移试验中显示出相似的运动性。尽管用针对单唾液酸-Gb 5的mAb RM 1处理大大增强了MCF-7/AZ细胞的侵袭性和运动性，但相同的RM 1处理对MCF-7/6没有影响。RM 1处理可激活MCF-7/AZ的cSrc和FAK，但不激活MCF-7/6。因此，MCF-7的恶性程度高度依赖于单唾液酸-Gb 5及其对GEM中cSrc和FAK的激活。
Two human mammary carcinoma cell variants, MCF‐7/AZ and MCF‐7/6, show the same composition in their glycosphingolipid‐enriched microdomain (GEM) with regard to globo‐series structures Gb3, Gb4, Gb5, monosialyl‐Gb5, GM2, and cSrc and FAK. Both variants are non‐invasive into collagen gel layer, and showed similar motility in wound migration assay. Whereas invasiveness and motility of MCF‐7/AZ cells were enhanced greatly by treatment with mAb RM1 directed to monosialyl‐Gb5, the same RM1 treatment had no effect on MCF‐7/6. cSrc and FAK of MCF‐7/AZ, but not MCF‐7/6, were activated by RM1 treatment. Thus, malignancy of MCF‐7 is highly dependent on monosialyl‐Gb5, and its activation of cSrc and FAK in GEM.