Differential expression of connective tissue growth factor gene in cutaneous fibrohistiocytic and vascular tumors
Differential expression of connective tissue growth factor gene in cutaneous fibrohistiocytic and vascular tumors
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DOI：
10.1111/j.1600-0560.1998.tb01706.x
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发表时间：
1998-03-01
影响因子：
1.7
通讯作者：
Takehara, K
中科院分区：
文献类型：
--
作者：
Igarashi, A;Hayashi, N;Takehara, K
结缔组织生长因子(CTGF)是即刻早期基因产物家族中的一员，可能在组织再生、伤口修复和皮肤纤维化过程中发挥重要作用。本研究用原位杂交法检测CTGF基因在间叶性肿瘤中的表达，并用免疫组织化学方法检测CD34抗原的表达。CTGF在9例皮肤纤维瘤成纤维细胞中均有表达，但7例隆突性皮肤纤维肉瘤(DFSP)和2例恶性纤维组织细胞瘤中有5例表达阴性。而CD34抗原仅在DFSP中表达。在血管肿瘤中，CTGF mRNA在化脓性肉芽肿中表达，而在血管肉瘤中不表达。此外，血管脂肪瘤和血管肌瘤内皮细胞表达CTGF mRNA，而静脉湖血管内皮细胞不表达。血管病变CD34表达均为阳性。其他来源的肿瘤CTGF基因表达均为阴性。我们的研究结果表明，良性成纤维细胞和/或血管内皮细胞在激活时具有表达CTGF mRNA的能力，但当它们达到恶性潜能时，这些细胞就失去了这种能力。因此，检测CTGF基因的表达可能有助于鉴别良、恶性间充质肿瘤，或确定结缔组织肿瘤的来源。
Connective tissue growth factor (CTGF) is a member of a family of immediate early gene products that may play an important role during tissue regeneration, wound repair and skin fibrosis. In this study, CTGF gene expression in mesenchymal tumors was investigated by in situ hybridization and CD34 antigen expression was studied by means of immunohistochemical staining. CTGF mRNA was expressed in fibroblasts of all nine dermatofibromas examined, but five of seven dermatofibrosarcoma protuberans (DFSP) or two cases of malignant fibrous histiocytoma were negative for its expression. In contrast, CD34 antigen was expressed only in DFSP. In vascular tumors, CTGF mRNA was expressed in pyogenic granuloma but not in angiosarcoma. In addition, the endothelial cells in angiolipoma and angioleiomyoma, but not in venous lake, expressed CTGF mRNA. These vascular lesions were all positive for CD34 expression. Tumors of other origins were negative for CTGF mRNA. Our findings indicated that benign fibroblasts and/or vascular endothelial cells have the capability to express CTGF mRNA when activated, but these cells lose this ability when they achieve malignant potency. Thus, examination of CTGF gene expression may be useful for differentiating between benign and malignant mesenchymal tumors, or to determine the origin of the tumors in connective tissue.