Different microRNA alterations contribute to diverse outcomes following EV71 and CA16 infections: Insights from high-throughput sequencing in rhesus monkey peripheral blood mononuclear cells
Different microRNA alterations contribute to diverse outcomes following EV71 and CA16 infections: Insights from high-throughput sequencing in rhesus monkey peripheral blood mononuclear cells
复制标题
不同的 microRNA 改变导致 EV71 和 CA16 感染后的不同结果：来自恒河猴外周血单核细胞高通量测序的见解
DOI：
10.1016/j.biocel.2016.10.011
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发表时间：
2016-12-01
影响因子：
4
通讯作者：
Li, Qihan
中科院分区：
文献类型：
--
作者：
Hui, Yajie;Song, Jie;Li, Qihan
肠道病毒71型（EV 71）和柯萨奇病毒A16型（CA 16）是手足口病（HFMD）的主要病原体。虽然这些病毒表现出遗传同源性，但两种病毒引起的临床表现存在一定差异。此外，导致这些差异的潜在机制仍不清楚。microRNA（miRNAs）参与许多生物学或病理学过程，包括宿主对病毒感染的应答。在这里，我们集中在不同的时间点感染EV 71和CA 16的恒河猴外周血单核细胞（PBMC）中的miRNA表达模式的差异，使用高通量测序。结果表明，106个已知和13个新发现的miRNAs在EV 71和CA 16感染的样本中表现出显著差异，其中32个用于靶点预测的关键miRNAs在EV 71和CA 16感染的样本中呈现相反的趋势。预测靶点的GO和通路分析显示富集了14个生物学过程、10个分子功能、8个细胞组分和104条通路。随后，构建了miRNA-transcription factors、miRNA-predicted targets、miRNA-pathways和miRNA-pathways的调控网络，揭示了miRNAs在感染阶段的复杂调控机制。最终，我们分析了参与免疫系统过程的预测靶标的分级GO类别，这表明EV 71和CA 16感染后的先天性和适应性免疫可能是显著不同的。总之，这份报告是第一个描述miRNA表达谱与EV 71和CA 16感染的PBMC使用高通量测序。我们的研究结果为进一步研究miRNA在EV 71和CA 16感染引起的不同免疫应答中的调控作用提供了有价值的基础。(C)2016作者爱思唯尔有限公司出版
Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are the predominant pathogens of hand, foot, and mouth disease (HFMD). Although these viruses exhibit genetic homology, the clinical manifestations caused by the two viruses have some discrepancies. In addition, the underlying mechanisms leading to these differences remain unclear. microRNAs (miRNAs) participate in numerous biological or pathological processes, including host responses to viral infections. Here, we focused on differences in miRNA expression patterns in rhesus monkey peripheral blood mononuclear cells (PBMCs) infected with EV71 and CA16 at various time points using high-throughput sequencing. The results demonstrated that 106 known and 13 novel miRNAs exhibited significant differences, and 32 key miRNAs among them for target prediction presented opposite trends in the EV71- and CA16-infected samples. GO and pathway analysis of the predicted targets showed enrichment in 14 biological processes, 10 molecular functions, 8 cellular components and 104 pathways. Subsequently, regulatory networks of miRNA-transcription factors, miRNA-predicted targets, miRNA-GOs and miRNA-pathways were constructed to reveal the complex regulatory mechanisms of miRNAs during the infection phase. Ultimately, we analysed hierarchical GO categories of the predicted targets involved in immune system processes, which indicated that the innate and adaptive immunity following EV71 and CA16 infections may be remarkably distinct. In conclusion, this report is the first describing miRNA expression profiles in PBMCs with EV71 and CA16 infections using high-throughput sequencing. Our findings could provide a valuable basis for further studies on the regulatory roles of miRNAs related to the different immune responses caused by EV71 and CA16 infections. (C) 2016 The Authors. Published by Elsevier Ltd.