Long noncoding RNA BC032469, a novel competing endogenous RNA, upregulates hTERT expression by sponging miR-1207-5p and promotes proliferation in gastric cancer
Long noncoding RNA BC032469, a novel competing endogenous RNA, upregulates hTERT expression by sponging miR-1207-5p and promotes proliferation in gastric cancer
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长非编码RNA BC032469是一种新型竞争性内源RNA，通过海绵miR-1207-5p上调hTERT表达并促进胃癌增殖
DOI：
10.1038/onc.2015.413
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发表时间：
2016-07-07
期刊：
影响因子：
8
通讯作者：
Yang, S-M
中科院分区：
文献类型：
--
作者：
Lu, M-H;Tang, B.;Yang, S-M
长链非编码RNA（lncRNA）正在成为基因调控的新参与者，并与癌症的发展有关。为探讨lncRNA在胃癌发生发展中的重要作用及其机制，本研究筛选了胃癌组织及相应癌旁组织中的lncRNA，并评价了其对胃癌的影响。在此，我们报道了一种新的lncRNA BC 032469在胃癌组织中高表达，并且上调与胃癌患者的肿瘤大小、分化差和生存期短相关。下调BC 032469导致体外和体内增殖的显著抑制。从机制上讲，BC 032469可以直接与miR-1207- 5 p结合，并有效地作为miR-1207- 5 p的海绵来调节hTERT的去抑制。因此，BC 032469可能作为一种ceRNA发挥作用，以损害miR-1207- 5 p依赖性hTERT下调，这表明它可能是一种临床上有价值的胃癌预后不良的生物标志物。
Long noncoding RNAs (lncRNAs) are emerging as new players in gene regulation and are associated with the development of cancers. To investigate the important role and mechanism of lncRNAs in the progression of gastric cancer, we screened lncRNAs in gastric cancer tissues and corresponding adjacent tissues, and assessed the effects on gastric cancer. Here, we report that BC032469, a novel lncRNA, expressed highly in gastric cancer tissues, and the upregulation was clinically associated with larger tumor size, poor differentiation and shorter survival of gastric cancer patients. Downregulation of BC032469 resulted in a significant inhibition of proliferation in vitro and in vivo. Mechanistically, BC032469 could directly bind to miR-1207-5p and effectively functioned as a sponge for miR-1207-5p to modulate the derepression of hTERT. Thus, BC032469 may function as a ceRNA to impair miR-1207-5p-dependent hTERT downregulation, suggesting that it may be clinically valuable as a poor prognostic biomarker of gastric cancer.