Different apoptotic activity and p21WAF1/CIP1, but not p27Kip1, expression in serrated adenomas as compared with traditional adenomas and hyperplastic polyps of the colorectum
Different apoptotic activity and p21WAF1/CIP1, but not p27Kip1, expression in serrated adenomas as compared with traditional adenomas and hyperplastic polyps of the colorectum
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DOI：
10.1007/s00432-003-0478-y
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发表时间：
2003-08-01
影响因子：
3.6
通讯作者：
Otani, Y
中科院分区：
文献类型：
--
作者：
Mitomi, H;Sada, M;Otani, Y
目的.锯齿状腺瘤（SA），其中包括一个广泛的病变谱，可以大致分为两个亚型：I型，密切模仿增生性息肉（HP），和II型，明确的腺瘤性肿瘤。我们的初步研究结果显示，他们之间的临床病理差异。本研究旨在探讨I型和II型SA与传统腺瘤（TA）和HP相比的凋亡活性和细胞周期调节蛋白p21（WAF 1/CIP 1）和p27（Kip 1）的表达。方法.在苏木精-伊红染色的标本中估计凋亡活性，并在62例SA（19例I型和43例II型）、50例TA和19例HP中确定p21（WAF 1/CIP 1）或p27（Kip 1）免疫反应性。在等间隔的隐窝区（上、中和下三分之一）中，每1，000个上皮细胞计数凋亡或免疫反应性细胞的数量（百分比）。结果II型SA的中间隐窝区的凋亡活性（中位数0.2%，四分位数范围0.1-0.5%）高于HP（0.1%，0.1- 0.2%，P
Purpose. Serrated adenomas (SAs), which include a wide spectrum of lesions, can be broadly divided into two subtypes: type I, closely mimicking hyperplastic polyps (HPs), and type II, unequivocal adenomatous tumor. Our preliminary findings showed clinicopathologic differences between them. The present study was conducted to investigate apoptotic activity and expression of the cell cycle regulator proteins p21(WAF1/CIP1) and p27(Kip1) in type I and II SAs, as compared with traditional adenomas (TAs) and HPs. Methods. Apoptotic activity was estimated in hematoxylin-eosin stained specimens, and p21(WAF1/CIP1) or p27(Kip1) immunoreactivity was determined in 62 SAs (19 type I and 43 type II), 50 TAs and 19 HPs. The numbers (percentages) of apoptotic or immunoreactive cells were counted per 1,000 epithelial cells in equally separated crypt zones (upper, middle, and lower thirds). Results. The apoptotic activity in the middle, but not the upper or lower crypt zone was higher in type II SAs (median 0.2%, interquartile range 0.1-0.5%) than in HPs (0.1%, 0.1-0.2%, P