Dietary vitamin D3 requirement of Wuchang bream (Megalobrama amblycephala)
Dietary vitamin D3 requirement of Wuchang bream (Megalobrama amblycephala)
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DOI：
10.1016/j.aquaculture.2014.10.049
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发表时间：
2015-01
期刊：
影响因子：
4.5
通讯作者：
Miao Linghong;Ge Xianping;Xie Jun;L. Bo;Wang Ke-bao;Zhu Jian;Ren Ming-chun;Zhou Qun-lan;Pan Liangkun;Chen Ruli
中科院分区：
文献类型：
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作者：
Miao Linghong;Ge Xianping;Xie Jun;L. Bo;Wang Ke-bao;Zhu Jian;Ren Ming-chun;Zhou Qun-lan;Pan Liangkun;Chen Ruli
进行了 90 天的饲养试验，以量化武昌鲂 (Megalobrama amblycephala) 的膳食维生素 D3 需求量。配制了六种等氮（34.39 ± 0.12%，DM）和异脂（6.07 ± 0.06%，DM）半纯化日粮，其中含有分级的维生素 D3 水平（0、0.50、1.00、2.00、4.00 和 8.00 IU/g 日粮）。饲喂试验结束后，测定生长性能、身体和肌肉成分、血浆和机体生理生化指标，包括钙、磷、胆固醇、甘油三酯、葡萄糖和胰岛素。基于SGR和FCR，采用折线回归估计法估算出五常鳊鱼的最佳膳食维生素D3需要量分别为5.43IU/g和4.97IU/g。除粗脂外，全身成分与膳食维生素 D3 水平无关，而肌肉粗蛋白、粗脂和灰分显着依赖于膳食维生素 D3 水平。血浆、全身和肌肉中的磷含量与膳食维生素 D3 水平无关。随着膳食维生素 D3 水平从饮食 0 增加到 1 IU/g，血浆中的钙含量增加，此后随着饮食 2、4 和 8 的增加而下降。饲喂含有 8 IU/g 维生素 D3 和 2 IU/g 维生素 D3 的鱼的血浆胆固醇、甘油三酯和胰岛素含量显着升高（P<0.05），而不同膳食维生素 D3 水平的鱼的葡萄糖含量没有显着差异（P>0.05）。
A 90-day feeding trial was conducted to quantify the dietary vitamin D3 requirement of Wuchang bream (Megalobrama amblycephala). Six isonitrogenous (34.39 ± 0.12%, DM) and isolipidic (6.07 ± 0.06%, DM) semi-purified diets were formulated to contain graded vitamin D3levels (0, 0.50, 1.00, 2.00, 4.00, and 8.00 IU/g diet). At the end of the feeding trial, the growth performance, body and muscle composition, plasma and body physiological and biochemical index, including calcium, phosphorus, cholesterol, triglycerides, glucose and insulin, were determined. Based on the SGR and FCR, the optimal dietary vitamin D3requirement of Wuchang bream was estimated to be 5.43 IU/g and 4.97 IU/g using broken line regression estimation method, respectively. Whole body composition was independent of dietary vitamin D3levels, except for crude lipid, while muscle crude protein, crude lipid and ash were significantly dependent on dietary vitamin D3levels. The phosphorus content in plasma, whole body and muscle were independent of dietary vitamin D3levels. The calcium content in plasma increased with increasing dietary vitamin D3level from diet 0 to 1 IU/g, and thereafter declined with 2, 4 and 8 diets. Significantly higher plasma cholesterol, triglycerides and insulin content were observed in fish fed diets containing 8 and 2 IU/g vitamin D3(P< 0.05), while no significant differences in glucose content were observed with different dietary vitamin D3levels (P> 0.05).