Comparative Small RNA Analysis of Pollen Development in Autotetraploid and Diploid Rice.
Comparative Small RNA Analysis of Pollen Development in Autotetraploid and Diploid Rice.
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同源四倍体和二倍体水稻花粉发育的小 RNA 比较分析
DOI：
10.3390/ijms17040499
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发表时间：
2016-04-12
影响因子：
5.6
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Li X;Shahid MQ;Wu J;Wang L;Liu X;Lu Y
microRNAs（miRNAs）在植物繁殖中起着重要作用。然而，同源四倍体水稻microRNAome分析的知识相当有限。本研究采用高通量测序技术对水稻二倍体和多倍体花粉发育过程中的miRNAomes进行了分析。在同源四倍体水稻中共检测到172个差异表达的miRNAs（DEM），其中57个miRNAs在同源四倍体水稻中特异表达。在172个DEM中，分别有115个和61个miRNA表达上调和下调。对DEM表达上调的靶基因进行Gene Ontology分析，发现其在减数分裂前期的转运和膜转运、减数分裂期的生殖和单小孢子期的核苷酸结合中富集。osa-miR 5788和osa-miR 1432 -5p_R+1在减数分裂中表达上调，其靶基因与减数分裂相关基因相互作用，可能参与了染色体行为相关基因的调控。在同源四倍体水稻花粉发育过程中发现了大量与转座因子相关的24 nt siRNA，而在二倍体水稻中则显著减少，表明24 nt siRNA可能在花粉发育过程中发挥作用。这些发现为理解同源四倍体水稻花粉发育过程中多倍体对导致花粉不育的小RNA表达模式的影响提供了基础。
MicroRNAs (miRNAs) play key roles in plant reproduction. However, knowledge on microRNAome analysis in autotetraploid rice is rather limited. Here, high-throughput sequencing technology was employed to analyze miRNAomes during pollen development in diploid and polyploid rice. A total of 172 differentially expressed miRNAs (DEM) were detected in autotetraploid rice compared to its diploid counterpart, and 57 miRNAs were specifically expressed in autotetraploid rice. Of the 172 DEM, 115 and 61 miRNAs exhibited up- and down-regulation, respectively. Gene Ontology analysis on the targets of up-regulated DEM showed that they were enriched in transport and membrane in pre-meiotic interphase, reproduction in meiosis, and nucleotide binding in single microspore stage. osa-miR5788 and osa-miR1432-5p_R+1 were up-regulated in meiosis and their targets revealed interaction with the meiosis-related genes, suggesting that they may involve in the genes regulation associated with the chromosome behavior. Abundant 24 nt siRNAs associated with transposable elements were found in autotetraploid rice during pollen development; however, they significantly declined in diploid rice, suggesting that 24 nt siRNAs may play a role in pollen development. These findings provide a foundation for understanding the effect of polyploidy on small RNA expression patterns during pollen development that cause pollen sterility in autotetraploid rice.