RAPID SEPARATION OF LOW-MOLECULAR WEIGHT SOLUTES FROM LIPOSOMES WITHOUT DILUTION
RAPID SEPARATION OF LOW-MOLECULAR WEIGHT SOLUTES FROM LIPOSOMES WITHOUT DILUTION
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DOI：
10.1016/0003-2697(78)90172-0
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
GOLDMAN, ID
中科院分区：
文献类型：
--
作者：
FRY, DW;WHITE, JC;GOLDMAN, ID
脂质体可以在Sephadex G-50的微型柱上与低MW溶质分离，所述微型柱由1或5 ml塑料注射器的筒制成。首先通过离心从Sephadex珠中除去过量的流体，并将截留的脂质体和游离溶质的混合物施加到柱床。重复离心，迫使脂质体材料通过柱进入试管，同时游离溶质定量保留在Sephadex中。该方法适用于各种溶质，带电和中性脂质体的回收率均为92-100%。该技术具有优于从脂质体中分离脂质体外溶质的其他方法的优点。可以在几分钟内同时处理许多样品，而无需稀释脂质体制剂。葡聚糖凝胶内未截留的溶质可以很容易地在小体积的水或缓冲液中回收。
Liposomes can be separated from low MW solutes on minicolumns of Sephadex G-50 made from the barrels of 1- or 5 ml plastic syringes. Excess fluid is first removed from the Sephadex beads by centrifugation and a mixture of liposomal entrapped and free solute is applied to the column bed. The centrifugation is repeated forcing the liposomal material through the column into a test tube while the free solute is quantitatively retained in the Sephadex. The procedure is applicable to a variety of solutes and 92-100% recovery is achieved for both charged and neutral liposomes. This technique has advantages over other methods for separating extraliposomal solutes from liposomes. Numerous samples can be processed simultaneously within minutes with no dilution of the liposomal preparation. Nonentrapped solute within the Sephadex can be easily recovered in a small volume of water or buffer.