Identification of environmentally stable QTL for resistance against Leptosphaeria maculans in oilseed rape (Brassica napus).
Identification of environmentally stable QTL for resistance against Leptosphaeria maculans in oilseed rape (Brassica napus).
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DOI：
10.1007/s00122-015-2620-z
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发表时间：
2016-01
期刊：
影响因子：
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通讯作者：
Delourme R
中科院分区：
文献类型：
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作者：
Huang YJ;Jestin C;Welham SJ;King GJ;Manzanares-Dauleux MJ;Fitt BD;Delourme R
6个稳定的抗病QTL。利用5个冬季油菜田间试验数据，通过QTL×X环境互作分析，鉴定出油菜黑斑病(褐斑病)。油菜茎腐病是由斑点钩端螺旋体引起的一种世界性病害。数量性状基因座(QTL)介导的甘蓝型油菜对黑斑病的抗性被认为是小种非特异性的，具有潜在的持久性。黑斑病抗性QTL的鉴定和评价对选育持久抗病油菜品种具有重要意义。以油菜定位群体为材料，对5个不同环境下的冬季油菜大田试验中的黑斑病抗性QTL进行检测。共检测到17个与田间数量抗性有关的QTL，共解释了51%的表型变异。每次试验检测到的QTL数目在2-9个之间，单个QTL解释了2-25%的表型变异。QTL与环境互作分析表明，其中6个QTL对环境因子的敏感性较低，因此被认为是稳定的QTL。与这些稳定的QTL连锁的标记对于在不同环境中选育对斑点落叶松的有效抗性具有重要价值，这将有助于该病的可持续管理。本文的在线版本(doi：10.1007/s00122-0152620-z)包含补充材料，授权用户可以使用。
Six stable QTL for resistance againstL. maculans(phoma stem canker) have been identified by QTL × environment interaction analysis using data from five winter oilseed rape field experiments. Phoma stem canker, caused by Leptosphaeria maculans, is a disease of worldwide importance on oilseed rape (Brassica napus). Quantitative trait loci (QTL)-mediated resistance against L. maculans in B. napus is considered to be race non-specific and potentially durable. Identification and evaluation of QTL for resistance to L. maculans is important for breeding oilseed rape cultivars with durable resistance. An oilseed rape mapping population was used to detect QTL for resistance against L. maculans in five winter oilseed rape field experiments under different environments. A total of 17 QTL involved in ‘field’ quantitative resistance against L. maculans were detected and collectively explained 51 % of the phenotypic variation. The number of QTL detected in each experiment ranged from two to nine and individual QTL explained 2–25 % of the phenotypic variation. QTL × environment interaction analysis suggested that six of these QTL were less sensitive to environmental factors, so they were considered to be stable QTL. Markers linked to these stable QTL will be valuable for selection to breed for effective resistance against L. maculans in different environments, which will contribute to sustainable management of the disease. The online version of this article (doi:10.1007/s00122-015-2620-z) contains supplementary material, which is available to authorized users.