ENDOGENOUS NONECOTROPIC PROVIRUSES MAPPED WITH OLIGONUCLEOTIDE PROBES FROM THE LONG TERMINAL REPEAT REGION
ENDOGENOUS NONECOTROPIC PROVIRUSES MAPPED WITH OLIGONUCLEOTIDE PROBES FROM THE LONG TERMINAL REPEAT REGION
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DOI：
10.1007/bf00389541
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发表时间：
1994-05-01
期刊：
影响因子：
2.5
通讯作者：
COFFIN, JM
中科院分区：
文献类型：
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作者：
FRANKEL, WN;COFFIN, JM
我们利用从三种非生态性小鼠白血病病毒的长末端重复序列（LTR）中提取的寡核苷酸探针，对C57BL/6J、DBA/2J和C3H/HeJ小鼠毒株的内源性原病毒进行了鉴定。在BXH和BXD重组自交系（RI）菌株中，前病毒-宿主DNA连接片段分离，大多数片段很容易定位到确定的染色体区域。大多数LTR片段似乎与先前用同一病毒类的寡核苷酸环境区探针绘制的原病毒相对应。至少有22个元素代表新的原病毒位点，其中不超过一半可能是单独的ltr，另外6个可能对应先前用特异性较低的杂交探针鉴定的原病毒。与先前用env探针鉴定的前病毒一起，LTR探针反应元件代表了小鼠基因组中大多数内源性小鼠白血病前病毒。
We characterized endogenous proviruses in C57BL/6J, DBA/2J, and C3H/HeJ mouse strains with oligonucleotide probes derived from long terminal repeat (LTR) sequences of three classes of nonecotropic murine leukemia virus. The segregation of proviral-host DNA junction fragments was followed in BXH and BXD recombinant inbred (RI) strain sets, and most fragments mapped readily to defined chromosomal regions. Most of the LTR fragments appear to correspond to proviruses mapped previously with oligonucleotide env region probes of the same viral class. At least 22 elements represent new proviral loci, no more than half of which may be solo LTRs, and an additional six may correspond to proviruses identified previously with less specific hybridization probes. Together with proviruses identified previously with env probes, the LTR probe-reactive elements represent the majority of endogenous murine leukemia proviruses in the mouse genome.