Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7.
Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7.
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DOI：
10.2147/cmar.s284650
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发表时间：
2021
影响因子：
3.3
通讯作者：
Han R
中科院分区：
文献类型：
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作者：
Ji WC;Bao GJ;Yang FW;Sun L;Han R
本研究旨在探讨lncRNA NR2F1-AS1和H19在肝细胞癌（HCC）中的表达及相关机制。将2018年2月至2019年8月来我院的42例HCC患者纳入研究组（RG）。同时，将46名健康对照者作为对照组（CG）。购买BEL-7402、Huh-7人肝癌细胞和HL-7702人正常肝细胞，检测HCC患者血清和组织中NR2F1-AS1和H19的水平。将pcDNA3.1-NR2F1-AS1、si-NR2F1-AS1、NC、pcDNA3.1-H19和si-H19转染至BEL-7402和Huh-7细胞中。通过qRT-PCR检测样本中NR2F1-AS1和H19的水平，通过WB检测细胞中凋亡相关蛋白的表达。分别通过CCK8、Transwell或流式细胞术检测细胞增殖、侵袭或凋亡。人肝癌细胞中NR2F1-AS1和H19水平较高，lncRNA NR2F1-AS1和lncRNA H19的AUC均>0.8。 lncRNA NR2F1-AS1 和 lncRNA H19 与 HCC 分期相关。转染pcDNA3.1-NR2F1-AS1、si-NR2F1-AS1、NC、pcDNA3.1-H19、si-H19 BEL-7402和Huh-7细胞后，沉默NR2F1-AS1和H19表达可促进细胞凋亡，抑制细胞生长，而沉默其过表达可抑制Huh-7细胞的EMT过程。 lncRNA NR2F1-AS1和lncRNA H19基因在HCC中异常表达。此外，两者还能抑制Huh-7细胞的EMT过程，有效促进细胞凋亡。
This research was designed to probe into the expression and related mechanism of lncRNA NR2F1-AS1 and H19 in hepatocellular carcinoma (HCC). Forty-two HCC patients who came to our hospital from February 2018 to August 2019 were included into a research group (RG). Meanwhile, 46 healthy controls were regarded as a control group (CG). BEL-7402, Huh-7 human hepatoma cells and HL-7702 human normal liver cells were purchased, and the NR2F1-AS1 and H19 levels in serum and tissues of HCC patients were detected. PcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19 and si-H19 were transfected into BEL-7402 and Huh-7 cells. The NR2F1-AS1 and H19 levels in samples were detected via qRT-PCR, and the expression of apoptosis-related proteins in cells was tested through WB. Cell proliferation, invasion, or apoptosis was detected by CCK8, Transwell or flow cytometry, respectively. The NR2F1-AS1 and H19 levels were high in human hepatoma cells, and AUCs of lncRNA NR2F1-AS1 and lncRNA H19 were both >0.8. The lncRNA NR2F1-AS1 and lncRNA H19 were associated with HCC staging. After transfection of pcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19, si-H19 BEL-7402 and Huh-7 cells, silencing NR2F1-AS1 and H19 expression can promote apoptosis and inhibit cell growth, while silencing their over-expression can inhibit the EMT process of Huh-7 cells. lncRNA NR2F1-AS1 and lncRNA H19 genes are abnormally expressed in HCC. Furthermore, the two can suppress the EMT process of Huh-7 cells and promote apoptosis effectively.