Nuclear Uptake of Gold Nanoparticles Deduced Using Dual-Angle X-Ray Fluorescence Mapping
Nuclear Uptake of Gold Nanoparticles Deduced Using Dual-Angle X-Ray Fluorescence Mapping
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DOI：
10.1002/ppsc.201900140
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发表时间：
2019-07-30
影响因子：
2.7
通讯作者：
Currell, Frederick
中科院分区：
文献类型：
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作者：
McCulloch, Aaron;Bennie, Lindsey;Currell, Frederick
对细胞核掺入金纳米颗粒（AuNPs）的研究通常受到常规成像技术所产生的模糊性的限制。事实上，这表明，迄今为止，没有明确的成像证据，这种摄取在整个细胞中，特别是在单一的纳米颗粒水平。尽管细胞核是真核生物中最重要的亚细胞区室，金是医学应用中最常用的金属纳米颗粒，但这种理解上的缺陷仍然存在。在这里，双角度X射线荧光被用来显示细胞核内单独分辨的纳米颗粒，发现它们被很好地分离，79%的核内群体是单分散的。这些发现对纳米医学具有重要意义，这里通过一个特定的例子说明了所观察到的AuNP产生的辐射效应的预测增强，发现核内剂量增强跨越近五个数量级。
Studies into the cell nucleus' incorporation of gold nanoparticles (AuNPs) are often limited by ambiguities arising from conventional imaging techniques. Indeed, it is suggested that to date there is no unambiguous imaging evidence for such uptake in whole cells, particularly at the single nanoparticle level. This shortcoming in understanding exists despite the nucleus being the most important subcellular compartment in eukaryotes and gold being the most commonly used metal nanoparticle in medical applications. Here, dual-angle X-ray flouresence is used to show individually resolved nanoparticles within the cell nucleus, finding them to be well separated and 79% of the intranuclear population to be monodispersed. These findings have important implications for nanomedicine, illustrated here through a specific exemplar of the predicted enhancement of radiation effects arising from the observed AuNPs, finding intranuclear dose enhancements spanning nearly five orders of magnitude.