Neuronal processing of noxious thermal stimuli mediated by dendritic Ca2+ influx in Drosophila somatosensory neurons
Neuronal processing of noxious thermal stimuli mediated by dendritic Ca2+ influx in Drosophila somatosensory neurons
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DOI：
10.7554/elife.12959
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发表时间：
2016-02-15
期刊：
影响因子：
7.7
通讯作者：
Usui, Tadao
中科院分区：
文献类型：
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作者：
Terada, Shin-Ichiro;Matsubara, Daisuke;Usui, Tadao
对引起组织损伤的有害刺激的适当反应对于生物体的生存是必不可少的。果蝇幼虫中的IV类神经元是负责热、机械和光感觉的多模态伤害感受器。重要的是，激活IV类引起不同的回避行为，这取决于输入。我们发现，有害的热刺激，但不是蓝光刺激，引起了一个独特的模式IV类，这是由高频率的尖峰列车和一个大的钙离子上升后的树突（钙离子瞬态）的停顿。这两种反应都依赖于两个TRPA通道和L型电压门控钙通道（LVGCC），表明温度感觉引起Ca2+内流。IV类神经元放电频率的急剧波动增强了强烈的热回避。我们假设Ca2+内流可能是编码特定模态的关键信号。
Adequate responses to noxious stimuli causing tissue damages are essential for organismal survival. Class IV neurons in Drosophila larvae are polymodal nociceptors responsible for thermal, mechanical, and light sensation. Importantly, activation of Class IV provoked distinct avoidance behaviors, depending on the inputs. We found that noxious thermal stimuli, but not blue light stimulation, caused a unique pattern of Class IV, which were composed of pauses after high frequency spike trains and a large Ca2+ rise in the dendrite (the Ca2+ transient). Both these responses depended on two TRPA channels and the L-type voltage-gated calcium channel (LVGCC), showing that the thermosensation provokes Ca2+ influx. The precipitous fluctuation of firing rate in Class IV neurons enhanced the robust heat avoidance. We hypothesize that the Ca2+ influx can be a key signal encoding a specific modality.