Validation of DGAT1-2 polymorphisms associated with oil content and development of functional markers for molecular breeding of high-oil maize
Validation of DGAT1-2 polymorphisms associated with oil content and development of functional markers for molecular breeding of high-oil maize
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DOI：
10.1007/s11032-011-9644-0
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Li, Jiansheng
中科院分区：
文献类型：
--
作者：
Chai, Yuchao;Hao, Xiaomin;Li, Jiansheng
编码酰基辅酶A：二酰基甘油酰基转移酶（DGAT1-2）的基因是控制玉米籽粒中含油量和油酸组成的关键数量性状基因座。在这里，我们对负责玉米地方品种组和 155 个自交系（35 个高油系和 120 个正常系）中的油变异的 DGAT1-2 区域进行了重新测序。高油 DGAT1-2 等位基因存在于大多数北弗林特和南马特群体中，但在八个玉米带马特开放授粉群体中的五个以及大多数早期自交系中不存在。高油 DGAT1-2 等位基因的丢失可能是由于 20 世纪初的遗传漂变造成的，当时选择了一些玉米带马齿种群来开发高产自交系。使用 155 个自交系，关联分析检测到两个基于 PCR 的功能标记（HO06 和 DGAT04；基于 DGAT1-2 多态性开发）对籽粒含油量和油酸组成的显着影响。良种杂种郑单958的亲本自交系Zheng58和Chang7-2通过与两个功能标记的标记辅助回交从高油品系By804转移有利等位基因。在BC5F2:3群体中，三种基因型（-/-、+/-和+/+）的含油量分别为3.37、4.20和4.61%（Zheng58受体系）和4.14、4.67和5.25%（Chang7-2受体系）。与轮回亲本相比，含有高油DGAT1-2等位基因的纯合子粒的含油量增加了27-37%。因此，这些功能标记可用于将高油 DGAT1-2 等位基因重新引入现代自交系中，通过标记辅助回交来增加含油量。
The gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1-2) is a key quantitative trait locus that controls oil content and oleic acid composition in maize kernels. Here we re-sequenced the DGAT1-2 region responsible for oil variation in a maize landrace set and in 155 inbred lines (35 high-oil and 120 normal lines). The high-oil DGAT1-2 allele was present in most Northern Flint and Southern Dent populations but was absent in five of eight Corn Belt Dent open-pollinated populations and in most of the earlier inbred lines. Loss of the high-oil DGAT1-2 allele possibly resulted from genetic drift in the early twentieth century when a few Corn Belt Dent populations were selected for the development of high-grain-yield inbred lines. Association analysis detected significant effects of two PCR-based functional markers (HO06 and DGAT04; developed based on DGAT1-2 polymorphisms) on kernel oil content and oleic acid composition using the 155 inbred lines. Zheng58 and Chang7-2, the parent inbred lines of elite hybrid Zhengdan958, were used to transfer the favorable allele from the high-oil line By804 using marker-assisted backcrossing with the two functional markers. In BC5F2:3 populations, oil content of the three genotypes (-/-, +/-, and +/+) was, respectively, 3.37, 4.20, and 4.61% (Zheng58 recipient line) and 4.14, 4.67, and 5.25% (Chang7-2 recipient line). Oil content of homozygous kernels containing the high-oil DGAT1-2 allele increased by 27-37% compared with recurrent parents. Hence, these functional markers can be used to re-introduce the high-oil DGAT1-2 allele into modern inbred lines for increased oil content through marker-assisted backcrossing.