Long non-coding RNA HULC affects the proliferation, apoptosis, migration, and invasion of mesenchymal stem cells
Long non-coding RNA HULC affects the proliferation, apoptosis, migration, and invasion of mesenchymal stem cells
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长链非编码RNA HULC影响间充质干细胞的增殖、凋亡、迁移和侵袭
DOI：
10.1177/1535370218804781
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发表时间：
2018-09-01
影响因子：
3.2
通讯作者：
Zhao, Guangfeng
中科院分区：
文献类型：
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作者：
Li, Xiujun;Wang, Jiali;Zhao, Guangfeng
进一步研究间充质干细胞维持生长和功能的分子机制对其临床应用具有重要意义。越来越多的证据表明，长链非编码rna （lncRNAs）在间充质干细胞的调控中发挥着重要作用。最近有报道称，在肝癌中高度上调（HULC），与另一个lncRNA MALAT-1一起，加速了肝癌干细胞的生长。MALAT-1在间充质干细胞中的调节作用已被研究。然而，HULC对间充质干细胞的作用尚不清楚。在本研究中，我们在脐带间充质干细胞中过表达HULC，并分析了间充质干细胞的细胞表型、增殖、凋亡、迁移、侵袭和分化。我们发现过表达的HULC通过促进细胞分裂和抑制细胞凋亡显著促进细胞增殖。hulc过表达的间充质干细胞比对照间充质干细胞迁移和侵袭更快。HULC对间充质干细胞的表型和分化无影响。此外，我们发现间充质干细胞中HULC的表达可以被几种炎症因子降低，包括tnf - α、tgf - β 1和R848。综上所述，我们的数据表明，HULC在间充质干细胞的生长和功能维持中起着至关重要的作用，而不影响分化。
Further studies on the molecular mechanisms of mesenchymal stem cells in the maintenance of growth and function are essential for their clinical application. Growing evidence has shown that long non-coding RNAs (lncRNAs) play an important role in the regulation of mesenchymal stem cells. Recently, it is reported that highly upregulated in liver cancer (HULC), with another lncRNA MALAT-1, accelerated liver cancer stem cell growth. The regulating role of MALAT-1 in mesenchymal stem cells has been investigated. However, the effects of HULC on the mesenchymal stem cells are unknown. In this study, we over-expressed HULC in mesenchymal stem cells derived from umbilical cord and analyzed the cell phenotypes, proliferation, apoptosis, migration, invasion and differentiation of mesenchymal stem cells. We found that overexpression of HULC significantly promotes cell proliferation through promoting cell division and inhibits cell apoptosis. HULC-overexpressed mesenchymal stem cells migrate and invade faster than control mesenchymal stem cells. HULC has no effect on phenotypes and differentiation of mesenchymal stem cells. Furthermore, we found that the expression of HULC in mesenchymal stem cells could be reduced by several inflammatory factors, including TNF-alpha, TGF-beta 1, and R848. Taken together, our data demonstrated that HULC has a vital role in the growth and function maintenance of mesenchymal stem cells without affecting differentiation.