Coexistence of tet(X4), mcr-1, and bla(NDM-5) in ST6775 Escherichia coli Isolates of Animal Origin in China.
Coexistence of tet(X4), mcr-1, and bla(NDM-5) in ST6775 Escherichia coli Isolates of Animal Origin in China.
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DOI：
10.1128/spectrum.00196-22
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发表时间：
2022-04-27
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
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携带多个抗性基因的病原菌的出现引起了人们的极大关注。尚未研究大肠埃希菌中同时存在的移动的耐药基因对替加环素、粘菌素和碳青霉烯类抗生素产生耐药性。本研究的目的是表征三个E.共携带泰特（X4）、mcr-1和blaNDM-5的大肠杆菌分离株。通过PCR、桑格测序、抗菌药物敏感性试验、结合试验、Illumina测序、纳米孔测序和生物信息学分析，鉴定并表征共携带泰特（X4）、mcr-1和blaNDM-5的分离株。三个E从中国鸽群中分离到携带泰特（X4）、mcr-1和blaNDM-5的大肠杆菌菌株。除恩诺沙星外，几乎所有抗菌药物均耐药。泰特（X4）和blaNDM-5可偶联到E. coliC 600，而mcr-1在3株分离株中均为非转移性。三个分离株属于序列类型6775（ST 6775），并且鸽子养殖场中存在携带泰特（X4）、mcr-1和blaNDM-5的分离株的克隆传播。遗传分析显示，Tn 6330介导的mcr-1位于染色体上，泰特（X4）位于IncFII质粒上，blaNDM-5位于IncX 3质粒上。我们首先对E.同时携带泰特（X4）、mcr-1和blaNDM-5的大肠杆菌分离株。应采取相关措施降低携带泰特（X4）、mcr-1和blaNDM-5的病原体的患病率。重要性替加环素和粘菌素被认为是治疗多重耐药（MDR）细菌感染的重要抗菌药物，例如由产碳青霉烯酶的肠杆菌科（CPE）引起的感染。大肠杆菌中可移动的移动的耐药基因对最后抗生素的耐药性。大肠杆菌仍然未知。在这里，我们描述了E。表型和遗传上共携带泰特（X4）、mcr-1和blaNDM-5的大肠杆菌菌株。耐药基因泰特（X4）、mcr-1和blaNDM-5位于转座子或质粒上，这些转座子或质粒是与这些重要耐药基因的捕获、积累和传播相关的移动的遗传元件。E.携带泰特（X4）、mcr-1和blaNDM-5的大肠杆菌分离株强调了采用“同一健康”方法监测不同环境中新型移动的耐药基因共存的重要性。应全面评估此类MDR病原体从动物传播至人类的风险。
Emergence of pathogens harboring multiple resistance genes incurs great concerns. Cooccurrence of mobile resistance genes conferring resistance to tigecycline, colistin, and carbapenems in Escherichia coli has not been investigated. This study aimed to characterize three E. coli isolates coharboring tet(X4), mcr-1, and blaNDM-5. Isolates coharboring tet(X4), mcr-1, and blaNDM-5 were identified and characterized by PCR, Sanger sequencing, antimicrobial susceptibility testing, conjugation assays, Illumina sequencing, nanopore sequencing, and bioinformatic analysis. Three E. coli isolates carrying tet(X4), mcr-1, and blaNDM-5 were identified from pigeons in China. They were resistant to almost all antimicrobials except enrofloxacin. tet(X4) and blaNDM-5 could be conjugated into E. coli C600, but mcr-1 was nontransferable in three isolates. Three isolates belonged to sequence type 6775 (ST6775), and clonal dissemination of isolates carrying tet(X4), mcr-1, and blaNDM-5 existed in the pigeon farm. Genetic analysis revealed that mcr-1 mediated by the Tn6330 was located on the chromosome, tet(X4) was located on the IncFII plasmid, and blaNDM-5 was located on the IncX3 plasmid. We first characterized the E. coli isolates carrying tet(X4), mcr-1, and blaNDM-5 simultaneously. Relevant measures should be taken to decrease the prevalence of pathogens carrying tet(X4), mcr-1, and blaNDM-5. IMPORTANCE Tigecycline and colistin are regarded as vital antimicrobials to treat multidrug-resistant (MDR) bacterial infections, such as that caused by carbapenemase-producing Enterobacteriaceae (CPE). Cooccurrence of mobile resistance genes conferring resistance to last-resort antimicrobials in E. coli remains unknown. Here, we characterized E. coli strains coharboring tet(X4), mcr-1, and blaNDM-5 phenotypically and genetically. Resistance genes tet(X4), mcr-1, and blaNDM-5 were located on transposons or plasmids that were mobile genetic elements related to the capture, accumulation, and dissemination of such important resistance genes. The emergence of E. coli isolates carrying tet(X4), mcr-1, and blaNDM-5 highlights the importance of monitoring the coexistence of novel mobile resistance genes in different settings with a One Health approach. Risk of transmission of such MDR pathogens from animals to humans should be evaluated comprehensively.
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