Bs1, a New Chimeric Gene Formed by Retrotransposon-Mediated Exon Shuffling in Maize
Bs1, a New Chimeric Gene Formed by Retrotransposon-Mediated Exon Shuffling in Maize
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DOI：
10.1104/pp.110.157420
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发表时间：
2010-07-01
期刊：
影响因子：
7.4
通讯作者：
Bureau, Thomas E.
中科院分区：
文献类型：
--
作者：
Elrouby, Nabil;Bureau, Thomas E.
转座子是所有真核生物基因组的主要组成部分。虽然转座子传统上被认为是有害突变的原因，但最近的证据表明，转座子可能在宿主基因多样化和进化中发挥作用。例如，通过逆转录元件进行宿主基因转导已经被认为是很常见的，并且有可能通过对现有序列的改组来创造新的嵌合基因。我们先前已经证明，玉米(Zea Mays subsp.)Mays)反转录转座子BS1转导了来自三个不同寄主基因的序列。在这里，我们提供的证据表明，这些转导事件导致了一种既可转录又可翻译的嵌合新基因的产生。BS1的表达受到严格的控制，发生在耳朵发育早期的一个狭窄的发育窗口。尽管所有与BS1相关的转导事件都发生在ZEA物种形成之前，但在驯化的玉米或其祖先Z.mays亚种的不同群体中，可能已经出现了编码BS1蛋白的完整的、不间断的开放阅读框架。小球虫。我们讨论了基于结构域保守的潜在功能，以及转导序列与其宿主基因对应物之间功能约束的证据。
Transposons are major components of all eukaryotic genomes. Although traditionally regarded as causes of detrimental mutations, recent evidence suggests that transposons may play a role in host gene diversification and evolution. For example, host gene transduction by retroelements has been suggested to be both common and to have the potential to create new chimeric genes by the shuffling of existing sequences. We have previously shown that the maize (Zea mays subsp. mays) retrotransposon Bs1 has transduced sequences from three different host genes. Here, we provide evidence that these transduction events led to the generation of a chimeric new gene that is both transcribed and translated. Expression of Bs1 is tightly controlled and occurs during a narrow developmental window in early ear development. Although all Bs1-associated transduction events took place before Zea speciation, a full uninterrupted open reading frame encoding the BS1 protein may have arisen in domesticated maize or in the diverse populations of its progenitor Z. mays subsp. parviglumis. We discuss potential functions based on domain conservation and evidence for functional constraints between the transduced sequences and their host gene counterparts.