Chromosomol DNA fragments from mouse cells exposed to an intercalating agent contain a 175-kdalton terminal polypeptide.
Chromosomol DNA fragments from mouse cells exposed to an intercalating agent contain a 175-kdalton terminal polypeptide.
复制标题
来自暴露于嵌入剂的小鼠细胞的染色体 DNA 片段含有 175 kdalton 末端多肽。
DOI：
--
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
R. Hancock
中科院分区：
文献类型：
--
作者：
R. Ralph;R. Hancock
将175千道尔顿（kDa）多肽共价结合到暴露于嵌入剂4 ′-[（9-吖啶基）-氨基]甲磺酰-间-茴香胺的小鼠细胞的染色体DNA片段上。电子显微镜显示DNA片段上的末端蛋白，其5 '末端被封闭。由于拓扑异构酶II多肽链的相对分子质量也约为175 kDa，并且拓扑异构酶II抑制剂防止嵌入剂诱导的DNA片段化，因此我们提出共价结合至DNA片段的5 ′-末端的多肽是衍生自频繁整合的拓扑异构酶II的多肽，其操作使由嵌入引起的扭转应力正常化。
A 175 kdalton (kDa) polypeptide is bound covalently to the chromosomal DNA fragments from mouse cells exposed to the intercalating agent 4'-[(9-acridinyl)-amino]methansulphon-m-anisidide. Electron microscopy shows a terminal protein on the DNA fragments, whose 5'-termini are blocked. Since the relative molecular mass of topoisomerase II polypeptide chains is also about 175 kDa and topoisomerase II inhibitors prevent intercalator-induced DNA fragmentation, we propose that the polypeptide bound covalently to the 5'-terminus of the DNA fragments is a polypeptide derived from frequently integrated topoisomerase II operating to normalize torsional stress resulting from intercalation.