Taxonomic and functional characterization of the rumen microbiome of Japanese Black cattle revealed by 16S rRNA gene amplicon and metagenome shotgun sequencing
Taxonomic and functional characterization of the rumen microbiome of Japanese Black cattle revealed by 16S rRNA gene amplicon and metagenome shotgun sequencing
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通过 16S rRNA 基因扩增子和宏基因组鸟枪法测序揭示日本黑牛瘤胃微生物组的分类学和功能特征
DOI：
10.1093/femsec/fiab152
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发表时间：
2021
影响因子：
4.2
通讯作者：
Hiroyuki Hirooka
中科院分区：
文献类型：
--
作者：
Yoshiaki Sato;Hiroaki Takebe;Kento Tominaga;Kazato Oishi;Hajime Kumagai;Takashi Yoshida;Hiroyuki Hirooka
本研究旨在确定日本黑阉牛瘤胃微生物群的分类和功能特征。研究饲喂相同饲粮的6头JB阉牛（14.7±1.44月龄）和6头JB sires × Holstein dam杂交（F1）阉牛（11.1±0.39月龄）瘤胃微生物组。基于16S rRNA基因测序，β多样性揭示了JB和F1瘤胃微生物群落结构的差异。霰弹枪测序显示琥珀酸原纤维杆菌和2个uminococcussp。与纤维素降解有关的蛋白质在JB型瘤胃中比F1型瘤胃中相对丰富。此外，16S rRNA基因的拷贝数为ofF。实时定量聚合酶链反应结果显示，肉牛瘤胃中琥珀酸原显著高于F1瘤胃。编码加速纤维素降解和半纤维素降解相关酶的基因在JB阉牛瘤胃中富集。AlthoughPrevotellaspp。在JB型和F1型瘤胃中均占主导地位，这些基因编码普氏菌的碳水化合物活性酶。可能在JB和F1之间有所不同。
This study aimed to determine the taxonomic and functional characteristics of the Japanese Black (JB) steer rumen microbiome. The rumen microbiomes of six JB steers (age 14.7 ± 1.44 months) and six JB sires × Holstein dams crossbred (F1) steers (age 11.1 ± 0.39 months), fed the same diet, were evaluated. Based on 16S rRNA gene sequencing, the beta diversity revealed differences in microbial community structures between the JB and F1 rumen. Shotgun sequencing showed thatFibrobacter succinogenesand twoRuminococcusspp., which are related to cellulose degradation were relatively more abundant in the JB steer rumen than in the F1 rumen. Furthermore, the 16S rRNA gene copy number ofF. succinogeneswas significantly higher in the JB steer rumen than in the F1 rumen according to quantitative real-time polymerase chain reaction analysis. Genes encoding the enzymes that accelerate cellulose degradation and those associated with hemicellulose degradation were enriched in the JB steer rumen. AlthoughPrevotellaspp. were predominant both in the JB and F1 rumen, the genes encoding carbohydrate-active enzymes ofPrevotellaspp. may differ between JB and F1.