Investigations on the disposition of oral doses of some water-insoluble pigments.
Investigations on the disposition of oral doses of some water-insoluble pigments.
复制标题
对某些水不溶性颜料口服剂量处置的研究。
DOI：
10.1007/bf01607481
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发表时间：
1984
影响因子：
2.7
通讯作者：
Hill,DL
中科院分区：
文献类型：
--
作者：
ElDareer,SM;Tillery,KF;Hill,DL
MATERIALS AND METHODSRed# 3 and Red# 23 were obtained from American Cyanamid, Wayne, NJ, and Yellow# 74 from Radian Corp., Austin, TX. Dosing suspensions of the three pigments were prepared in corn oil. Spectrophotometric assays were performed to determine the concentration of the dosing solutions and to assure that the preparations were homogeneous and stable during the time of dosing. For such assays, the pigments were dissolved in methylene chloride. Doses administered were determined to be as follows: Red# 3, 11.8 mg/kg; Red# 23, 5.2 mg/kg; and Yellow# 74, 12.6 mg/kg. The lower dose for Red# 23 was necessitated by its extreme insolubility in methylene chloride.Chemical purity was assessed by high-pressure liquid chromatographic anal~ with a Waters series ALC 200 instrument equipped with a model 440 absorbance detector and with a column of Spherisorb ODS, 10-micron particle size. Detection was at 254 nm. Eluting solvents were acetonitrile: water (7: 3, v/v) for Red# 3 and Yellow# 74 and acetonitrile: water (17: 3, v/v) for Red# 23. Purities were determined to be as follows: Red# 3, 94.7%; Red# 23, 84.6%; and Yellow# 74, 89.1%. Recoveries were calculated on the basis of the amount of authentic compound administered.
