Advancement through epidermis using tape stripping technique and Reflectance Confocal Microscopy
Advancement through epidermis using tape stripping technique and Reflectance Confocal Microscopy
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DOI：
10.1038/s41598-019-48698-w
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发表时间：
2019-08-21
影响因子：
4.6
通讯作者：
Clausen, Maja-Lisa
中科院分区：
文献类型：
--
作者：
Olesen, Caroline Meyer;Fuchs, Christine Sofie Krohn;Clausen, Maja-Lisa
胶带剥离技术在有关皮肤屏障功能的研究中越来越多地使用。然而，在不同的研究中，带子的数目是不同的，关于进入角质层/表皮与带子数目有关的文献很少。这项先导性研究的目的是评估使用胶带剥离技术在健康志愿者身上通过表皮的进展。研究对象为10名健康志愿者。从所有志愿者的前臂掌侧4个相邻皮肤区连续取0、5、15和35张胶带(D-Squame)，然后用反射共聚焦显微镜(RCM)对这4个皮肤区的表皮厚度进行评估。使用Squame扫描来确定蛋白质的去除量。所有志愿者在35条胶带后角质层被完全去除。进入表皮的主要是通过前15条胶带，去除了总表皮的25%，而35条胶带去除了33%的表皮。每条胶带的蛋白质去除量随着深度的增加而减少。根据所取胶带的数量，有关进入表皮的信息是向前迈出的重要一步。从不同层次的表皮获取样本的可能性可能会导致对皮肤屏障属性的更好理解。
The tape stripping technique is increasingly used in research regarding skin barrier function. However, number of tape strips varies between studies, and literature considering advancement into stratum corneum/epidermis in relation to number of tape strips is scarce. The aim of this pilot study was to assess the advancement through epidermis using tape stripping technique in healthy volunteers. A total of ten healthy volunteers were included. From all volunteers 0, 5, 15 and 35 consecutive tape strips (D-squame) were taken from four adjacent skin areas on the middle volar forearm, followed by Reflectance Confocal Microscopy (RCM) of the four areas to assess epidermal thickness. Squame Scan was used to determine amount of protein removed. Stratum corneum was completely removed in all volunteers after 35 tape strips. Advancement into epidermis was predominantly achieved by the first 15 tape strips, removing 25% of the total epidermis, whereas 35 tape strips removed 33% of epidermis. Protein removal per tape decreased with increasing depth. Information on advancement into the epidermis according to number of tape strips taken, is a significant step forward. The possibility to obtain samples from different layers of epidermis may lead to an improved understanding of skin barrier properties.