Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem Cells
Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem Cells
复制标题
胚胎干细胞神经分化衍生的脱细胞基质增强牙囊干细胞的神经发生潜力。
DOI：
10.1016/j.joen.2016.10.033
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发表时间：
2017-03-01
影响因子：
4.2
通讯作者：
Zhang, Chengfei
中科院分区：
文献类型：
--
作者：
Heng, Boon Chin;Gong, Ting;Zhang, Chengfei
前言：牙囊干细胞来源于胚胎神经嵴，具有神经源性潜能。本研究旨在探讨人胚胎干细胞（human embryonic stem cells，hESCs）神经发生来源的脱细胞基质（decellularized matrix substrate，NSC-DECM）能否促进DFSCs向神经元分化。将接种在NSC-DECM、Geltrex和组织培养聚苯乙烯（TCPS）上的DFSC在21天的时间内进行神经诱导。在第7、14和21天的时间点，采用定量实时聚合酶链反应分析DFSC的早期/中期（Musashi 1、PAX 6、NSE和β 111-微管蛋白）和成熟/晚期（NGN 2、NeuN、NFM和MASH 1）神经标志物的表达。免疫细胞化学检测β 111-微管蛋白，PAX 6，和NGN 2的表达DFSCs在第7天的神经induction.Results：定量RT-PCR显示，表达PAX 6，Musashi 1，β III-微管蛋白，NSE，NGN 2，和NFM的DFSCs上NSCDECM增强无论是Geltrex或TCPS组。免疫细胞化学显示，在NSC-DECM组DFSCs显示更强烈的染色β III-微管蛋白，PAX 6，和NGN 2的表达，连同更多的神经突生长和细长的形态，与Geltrex或TCPS.Conclusions相比：DECM来源于hESCs的神经发生可以增强DFSCs的神经发生潜力。
Introduction: Dental follicle stem cells (DFSCs) possess neurogenic potential because they originate from the embryonic neural:crest. This study investigated whether neural differentiation of DFSCs can be enhanced by culture on decellularized matrix substrata (NSC-DECM) derived from neurogenesis of human embryonic stem cells (hESCs).Methods: The hESCs were differentiated into neural stem cells (NSCs), and NSC-DECM was extracted from confluent monolayers of NSCs through treatment with deionized water. DFSCs seeded on NSC-DECM, Geltrex, and tissue culture polystyrene (TCPS) were subjected to neural induction during a period of 21 days. Expression of early/intermediate (Musashi1, PAX6, NSE, and beta 111-tubulin) and mature/late (NGN2, NeuN, NFM, and MASH1) neural markers by DFSCs was analyzed at the 7-, 14-, and 21-day time points with quantitative real-time polymerase chain reaction. Immunocytochemistry for detection of beta 111-tubulin, PAX6, and NGN2 expression by DFSCs on day 7 of neural induction was also carried out.Results: Quantitative RT-PCR showed that expression of PAX6, Musashi1, beta llI-tubulin, NSE, NGN2, and NFM by DFSCs was enhanced on NSCDECM versus either the Geltrex or TCPS groups. Immunocytochemistry showed that DFSCs in the NSC-DECM group displayed more intense staining for beta lll-tubulin, PAX6, and NGN2 expression, together with more neurite outgrowths and elongated morphology, as compared with either Geltrex or TCPS.Conclusions: DECM derived from neurogenesis of hESCs can enhance the neurogenic potential of DFSCs.