Quiescent neuronal progenitors are activated in the juvenile guinea pig lateral striatum and give rise to transient neurons
Quiescent neuronal progenitors are activated in the juvenile guinea pig lateral striatum and give rise to transient neurons
复制标题
DOI：
10.1242/dev.107987
复制
发表时间：
2014-11-01
期刊：
影响因子：
4.6
通讯作者：
Peretto, Paolo
中科院分区：
文献类型：
--
作者：
Luzzati, Federico;Nato, Giulia;Peretto, Paolo
在成人大脑中，活性干细胞是位于海马室下区（SVZ）和齿状回（DG）的星形胶质细胞的一个子集。静止的神经元祖细胞是否存在于其他脑区尚不清楚。在这里，我们描述了一种新的神经系统的外囊和侧纹状体（EC-LS）的幼年豚鼠出生时是静止的，但在断奶时变得活跃。在这一地区的神经发生的激活伴随着出现的神经原性的利基在腹侧EC的特点是链的神经母细胞，中间体样祖细胞和胶质细胞表达标记的未成熟星形胶质细胞。与SVZ和DG的神经源性星形胶质细胞一样，后者的细胞表现出缓慢的增殖速率，并保留BrdU标记长达65天，这表明它们是EC-LS神经源性系统的主要祖细胞。将GFP标记的慢病毒载体注射到新生动物的SVZ和EC-LS中证实了新的LS成神经细胞来源于局部祖细胞的激活，并进一步支持了它们的星形胶质细胞性质。新生的EC-LS神经元存在短暂，并没有作出贡献的神经元添加或替代。然而，他们表达Sp 8，并表现出强烈的向性白色物质束，其中他们收购了复杂的形态。由于这些原因，我们提出，EC-LS神经母细胞代表一种新的纹状体细胞类型，可能与那些在胚胎期调节纤维束发育的瞬时中间神经元群体有关。
In the adult brain, active stem cells are a subset of astrocytes residing in the subventricular zone (SVZ) and the dentate gyrus (DG) of the hippocampus. Whether quiescent neuronal progenitors occur in other brain regions is unclear. Here, we describe a novel neurogenic system in the external capsule and lateral striatum (EC-LS) of the juvenile guinea pig that is quiescent at birth but becomes active around weaning. Activation of neurogenesis in this region was accompanied by the emergence of a neurogenic-like niche in the ventral EC characterized by chains of neuroblasts, intermediate-like progenitors and glial cells expressing markers of immature astrocytes. Like neurogenic astrocytes of the SVZ and DG, these latter cells showed a slow rate of proliferation and retained BrdU labeling for up to 65 days, suggesting that they are the primary progenitors of the EC-LS neurogenic system. Injections of GFP-tagged lentiviral vectors into the SVZ and the EC-LS of newborn animals confirmed that new LS neuroblasts originate from the activation of local progenitors and further supported their astroglial nature. Newborn EC-LS neurons existed transiently and did not contribute to neuronal addition or replacement. Nevertheless, they expressed Sp8 and showed strong tropism for white matter tracts, wherein they acquired complex morphologies. For these reasons, we propose that EC-LS neuroblasts represent a novel striatal cell type, possibly related to those populations of transient interneurons that regulate the development of fiber tracts during embryonic life.