Amplification of CRAC current by STIM1 and CRACM1 (Orai1)
Amplification of CRAC current by STIM1 and CRACM1 (Orai1)
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DOI：
10.1038/ncb1435
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发表时间：
2006-07-01
影响因子：
21.3
通讯作者：
Kinet, Jean-Pierre
中科院分区：
文献类型：
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作者：
Peinelt, Christine;Vig, Monika;Kinet, Jean-Pierre
在许多细胞中，细胞内钙储存的消耗激活了储存操作的钙通过质膜进入。内质网中假定的钙传感器STIM1和质膜中的钙释放活化钙（CRAC）调节剂CRACM1（也称为Orai1）最近被证明对控制储存操作的CRAC电流（I-CRAC）至关重要（1-4）。然而，任何一种蛋白的个体过表达都不能显著扩增I-CRAC。在这里，我们展示了STIM1和CRACM1在功能上相互作用。这两种蛋白的过表达大大增强了I-CRAC，表明STIM1和CRACM1相互限制存储操作电流，CRACM1可能是长期寻找的CRAC通道。
Depletion of intracellular calcium stores activates store-operated calcium entry across the plasma membrane in many cells. STIM1, the putative calcium sensor in the endoplasmic reticulum, and the calcium release-activated calcium ( CRAC) modulator CRACM1 ( also known as Orai1) in the plasma membrane have recently been shown to be essential for controlling the store-operated CRAC current ( I-CRAC)(1-4). However, individual overexpression of either protein fails to significantly amplify I-CRAC. Here, we show that STIM1 and CRACM1 interact functionally. Overexpression of both proteins greatly potentiates I-CRAC, suggesting that STIM1 and CRACM1 mutually limit store-operated currents and that CRACM1 may be the long-sought CRAC channel.