Self-renewal of factor-dependent hemopoietic progenitor cell-lines derived from long-term bone marrow cultures demonstrates significant mouse strain genotypic variation.
Self-renewal of factor-dependent hemopoietic progenitor cell-lines derived from long-term bone marrow cultures demonstrates significant mouse strain genotypic variation.
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来自长期骨髓培养物的因子依赖性造血祖细胞系的自我更新表现出显着的小鼠品系基因型变异。
DOI：
10.1002/jss.400130409
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Greenberger,JS
中科院分区：
文献类型：
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作者：
Greenberger,JS
从C57 Ks/J小鼠建立的长期骨髓培养物已显示自发释放内源性嗜亲性RNA C型病毒（逆转录病毒）。C57 Ks/J骨髓培养物产生粒细胞-巨噬细胞祖细胞（GM-CFUc）和未成熟和成熟粒细胞超过45周。相比之下，NIH Swiss小鼠骨髓培养物在25-35周内未能释放可检测到的亲嗜性病毒并产生GM-CFUc和粒细胞，并在体外建立了WEHI-3条件培养基（CM）依赖性细胞系，但未建立永久性细胞系。为了确定病毒和/或细胞基因是否调节C57 Ks/J骨髓培养物的寿命，从（NIH‐Swiss × C57 Ks/J）F1杂交、F2杂交和（NIH Swiss × C57 Ks/J）× NIH Swiss回交世代的骨髓建立培养物组。每周测量各培养物中内源性嗜亲性病毒的释放，以及58周期间未成熟粒细胞和GM-CFUc的产生持续时间。结果证明了长期体外造血的长寿遗传的复杂模式。寿命延长与C57 Ks/J N嗜性病毒的可检测复制并不绝对相关。
Long‐term bone marrow cultures established from C57Ks/J mice have been shown to spontaneously release endogenous ecotropic RNA type‐C virus (retrovirus). C57Ks/J marrow cultures produced granulocyte‐macrophage progenitor cells (GM‐CFUc) and immature and mature granulocytes for over 45 weeks. In contrast, NIH Swiss mouse marrow cultures failed to release detectable ecotropic virus and generated GM‐CFUc and granulocytes for 25–35 weeks and established WEHI‐3 conditioned medium (CM) dependent cell lines in vitro and did not establish permanent cell lines. To determine whether viral and/or cellular genes regulated the longevity of C57Ks/J marrow cultures, groups of cultures were established from the marrow of (NIH‐Swiss × C57Ks/J) F1 hybrid, F2 hybrid, and (NIH Swiss × C57Ks/J) X NIH Swiss backcross generations. Release of endogenous ecotropic virus was measured weekly in each culture as was the duration of production of immature granulocytic cells and GM‐CFUc over a 58‐week period. The results demonstrated a complex pattern of inheritance of longevity of long‐term in vitro hemopoiesis. Increased longevity did not absolutely correlate with detectable replication of the C57Ks/J N‐tropic virus.