Effect of brefeldin A and the dynamics of the actin plate on cytokinesis of zygotes in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)
Effect of brefeldin A and the dynamics of the actin plate on cytokinesis of zygotes in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)
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布雷菲德菌素 A 和肌动蛋白板动态对褐藻 Silvetia babingtonii（墨角藻目，褐藻纲）受精卵胞质分裂的影响
DOI：
10.1080/09670262.2018.1501516
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发表时间：
2018
影响因子：
2.4
通讯作者：
Motomura Taizo
中科院分区：
文献类型：
--
作者：
Nagasato Chikako;Motomura Taizo
在褐藻的胞质分裂中，肌动蛋白丝在有丝分裂后从中心体中出现的微管（MT）的交叉区域呈现出板状。肌动蛋白板本身的功能仍然是未知的。为了阐明肌动蛋白板，MTs和膜融合之间的关系，而不诱导细胞骨架解聚，布雷菲德菌素A（BFA），它可以防止从高尔基体产生的小泡的效果，在Silvetia babingtonii受精卵中进行了检查。与对照组相比，BFA处理的受精卵有丝分裂开始时间稍有延迟。几乎所有的受精卵细胞质分裂的进程受到抑制的BFA处理。超微结构观察表明，连续用BFA处理后，高尔基体池变得破碎或卷曲，合子间的胞质分裂处于抑制状态。下一个细胞周期在胞质分裂完成之前开始。虽然肌动蛋白板的外观没有受到BFA处理的干扰，但在第一和第二细胞周期之间的过渡期间肌动蛋白板的行为可以分为两种模式：在下一个细胞周期开始时它是不可见的，或者即使下一个细胞周期已经开始，它的一部分仍然存在。在后一种情况下，肌动蛋白板的一部分似乎与新的部分形成的细胞分隔膜，并从中心体的MT结合到它。肌动蛋白板完全消失在下一个有丝分裂，然后重新出现在中间区域的四个子核。本研究的结果表明，在BFA处理下，肌动蛋白板持续到下一个有丝分裂期开始之前，此时存在新的不完整的细胞分隔膜，并且MT持续肌动蛋白板直到下一次有丝分裂。
In the cytokinesis of brown algae, actin filaments appear like a plate at the intersecting region of microtubules (MTs) that emerge from the centrosomes after mitosis. The function of the actin plate itself is still unknown. To elucidate the relationship between the actin plate, MTs and membrane fusion, without inducing cytoskeleton depolymerization, the effect of brefeldin A (BFA), which prevents the production of vesicles from Golgi bodies, was examined in zygotes ofSilvetia babingtonii. The beginning of mitosis was slightly delayed in zygotes under BFA compared with the controls. Almost all zygotes were inhibited for the progression of cytokinesis by BFA treatment. Ultrastructural observations showed that Golgi cisternae became fragmented or curled following continuous treatment with BFA, and the inhibitory status of cytokinesis between zygotes. The next cell cycle started before cytokinesis was completed. Although the appearance of the actin plate was not disturbed by BFA treatment, the behaviour of the actin plate during the transition between the first and second cell cycles could be classified into two patterns: it was either invisible upon the initiation of the next cell cycle, or a portion of it remained even though the next cell cycle had begun. In the latter case, a part of the actin plate seemed to associate with the new partially formed cell partition membrane, and MTs from the centrosomes were bound to it. The actin plate completely disappeared in the next mitosis, then re-emerged in the middle area of the four daughter nuclei. The results of the present study indicated that, under BFA treatment, the actin plate persisted until just before the beginning of the next mitotic phase, when the new, incomplete cell partition membrane was present, and MTs sustained the actin plate until next mitosis.