Expression of the Hsp83 gene in response to diapause and thermal stress in the moth Sesamia nonagrioides
Expression of the Hsp83 gene in response to diapause and thermal stress in the moth Sesamia nonagrioides
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DOI：
10.1111/j.1365-2583.2009.00922.x
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发表时间：
2009-12-01
影响因子：
2.6
通讯作者：
Kourti, A.
中科院分区：
文献类型：
--
作者：
Gkouvitsas, T.;Kontogiannatos, D.;Kourti, A.
从玉米茎螟Sesamia nonagrioides中克隆了一个热休克蛋白83（Hsp 83）的全长cDNA，命名为SnoHsp 83。基因组分析表明，SnoHsp 83基因是唯一的。发现SnoHsp 83 cDNA的大小约为2.6kb。推导的多肽由717个氨基酸残基组成，分子量为82.6 kDa。它包含了所有的高度保守的氨基酸基序，表征的hsp 90家族的胞质成员。我们研究了SnoHsp 83基因在滞育和热/冷胁迫下的表达。SnoHsp 83在非滞育幼虫中组成型表达，并被热诱导15倍。在滞育条件下，当额外的幼虫蜕皮发生时，SnoHsp 83显示出与SnoHsc 70相似的模式。我们的研究结果表明，SnoHsp 83基因可能参与了两次蜕皮之间发生的发育过程。
A full-length Hsp83, named SnoHsp83, cDNA from the corn stalk borer, Sesamia nonagrioides, was cloned and sequenced. Genomic analysis showed that the SnoHsp83 gene is unique. The size of the SnoHsp83 cDNA was found to be approximately 2.6 kb. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82.6 kDa. It contained all the highly conserved amino acid motifs that characterize the cytosolic members of the hsp90 family. We investigated the expression of SnoHsp83 gene in response to diapause and heat/cold stress. SnoHsp83 is constitutively expressed in non-diapausing larvae and is induced 15-fold by heat. SnoHsp83 displays a similar pattern to SnoHsc70 under diapause conditions, when extra larval moults occur. Our results indicate that the SnoHsp83 gene could be involved in the developmental process that occurs between two moults.