Volatile organic compounds in the exhaled breath of young patients with cystic fibrosis
Volatile organic compounds in the exhaled breath of young patients with cystic fibrosis
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DOI：
10.1183/09031936.06.00085105
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发表时间：
2006-05-01
影响因子：
24.3
通讯作者：
Koppman, R
中科院分区：
文献类型：
--
作者：
Barker, M;Hengst, M;Koppman, R
呼气中的炎症介质作为非侵入性疾病标志物正受到越来越多的医学关注。在此背景下，挥发性有机化合物已被研究，但临床信息和方法标准有限。测定了20例囊性纤维化患者和20例健康对照(8-29岁)重复呼气样本中乙烷、丙烷、正戊烷、甲醇、乙醇、异丙醇、丙酮、异戊二烯、苯、甲苯、二甲基硫化物(DMS)和柠檬烯的水平。每个人采集了三个末端呼出的空气样本和一个环境空气样本，并在定制的气相色谱系统上进行了分析。受试者内的变异系数在9%到34%之间，碳氢化合物呼气水平受到吸入浓度的影响。囊性纤维化患者的肺泡戊烷梯度高于健康对照组(0.36 ppb比0.21 ppb)，并与1秒内的用力呼气量成反比；在肺恶化患者中观察到最高值(0.73 ppb比0.24 ppb)。囊性纤维化患者的DMS产量也较低(3.9 ppb对7.6 ppb)。乙烷和其余物质的组间差异不显著。结论：挥发性有机化合物的化学呼气分析是可行的，并可能在囊性纤维性肺疾病的炎症过程的无创性诊断和随访中具有潜力。
Inflammatory mediators in the exhaled breath are receiving growing medical interest as noninvasive disease markers. Volatile organic compounds have been investigated in this context, but clinical information and methodological standards are limited.The levels of ethane, propane, n-pentane, methanol, ethanol, 2-propanol, acetone, isoprene, benzene, toluene, dimethyl sulphide (DMS) and limonene were measured in repeated breath samples from 20 cystic fibrosis patients and 20 healthy controls (aged 8-29 yrs). Three end-exhaled and one ambient air sample were collected per person and analysed on a customised gas chromatography system.Intra-subject coefficients of variation ranged between 9 and 34%, and hydrocarbon breath levels were influenced by their inspired concentrations. The alveolar gradient for pentane was higher in cystic fibrosis patients than in healthy controls (0.36 versus 0.21 ppb) and inversely proportional to forced expiratory volume in one second; highest values were observed in patients with pulmonary exacerbations (0.73 versus 0.24 ppb). Cystic fibrosis patients also exhibited a lower output of DMS (3.9 versus 7.6 ppb). Group differences were not significant for ethane and the remaining substances.It was concluded that chemical breath analysis for volatile organic compounds is feasible and may hold potential for the noninvasive diagnosis and follow-up of inflammatory processes in cystic fibrosis lung disease.