The SERK1 receptor-like kinase regulates organ separation in Arabidopsis flowers.
The SERK1 receptor-like kinase regulates organ separation in Arabidopsis flowers.
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DOI：
10.1111/j.1365-313x.2010.04194.x
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发表时间：
2010-06-01
期刊：
影响因子：
--
通讯作者：
Liljegren SJ
中科院分区：
文献类型：
--
作者：
Lewis MW;Leslie ME;Fulcher EH;Darnielle L;Healy PN;Youn JY;Liljegren SJ
通过对调控拟南芥花器官分离途径的新成分的敏化筛选，我们发现富含亮氨酸重复序列的受体样蛋白1(SERK1)作为脱落的负调控因子。在没有功能性NEVERSHED(Nev)的花中，SERK1的突变主要拯救脱落，Nev是花器官脱落所需的一种ADP核糖化因子GTP酶激活蛋白。我们以前报道过，Nev突变花中高尔基体的组织结构和跨高尔基体网络(TGN)的位置发生了变化。SERK1的破坏恢复了Nev花中高尔基体的结构和TGN的紧密联系，这表明这些细胞器中的缺陷可能是导致脱落障碍的原因。我们还发现，与野生型相比，Nev SERK1花的离区较大。以前在植物中观察到了类似的表型，表达器官分离所需的假定配体--花序缺陷型脱落(IDA)，这表明通过IDA及其建议的受体HAESA和HAESA-LIKE2的信号在Nev SERK1离区细胞中可能被解除调控。我们的研究表明，SERK1除了在雄蕊发育和油菜素类固醇感受中发挥先前所特有的作用外，还在调节器官脱落过程中发生的细胞黏附丧失方面发挥独特的作用。
Through a sensitized screen for novel components of pathways regulating organ separation in Arabidopsis flowers, we have found that the leucine-rich repeat receptor-like kinase SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) acts as a negative regulator of abscission. Mutations in SERK1 dominantly rescue abscission in flowers without functional NEVERSHED (NEV), an ADP-ribosylation factor GTPase-activating protein required for floral organ shedding. We previously reported that the organization of the Golgi apparatus and location of the trans-Golgi network (TGN) are altered in nev mutant flowers. Disruption of SERK1 restores Golgi structure and the close association of the TGN in nev flowers, suggesting that defects in these organelles may be responsible for the block in abscission. We have also found that the abscission zones of nev serk1 flowers are enlarged compared to wild-type. A similar phenotype was previously observed in plants constitutively expressing a putative ligand required for organ separation, INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), suggesting that signaling through IDA and its proposed receptors, HAESA and HAESA-LIKE2, may be deregulated in nev serk1 abscission zone cells. Our studies indicate that in addition to its previously characterized roles in stamen development and brassinosteroid perception, SERK1 plays a unique role in modulating the loss of cell adhesion that occurs during organ abscission.