Low complex I content explains the low hydrogen peroxide production rate of heart mitochondria from the long-lived pigeon, Columba livia
Low complex I content explains the low hydrogen peroxide production rate of heart mitochondria from the long-lived pigeon, Columba livia
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DOI：
10.1111/j.1474-9726.2009.00538.x
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发表时间：
2010-02-01
期刊：
影响因子：
7.8
通讯作者：
Brand, Martin D.
中科院分区：
文献类型：
--
作者：
Lambert, Adrian J.;Buckingham, Julie A.;Brand, Martin D.
在一系列脊椎动物物种中，已知最大寿命与线粒体过氧化氢产量之间存在负相关。在本报告中，我们研究了长寿物种（鸽子）与体重相似的短命物种（大鼠）心脏线粒体过氧化氢生成率低的潜在生化基础。过氧化氢排出率的差异不能用超氧化物歧化酶活性或过氧化氢去除能力的差异来解释。在琥珀酸氧化过程中，不同物种间过氧化氢生成速率的差异局限于复合物i中Delta pH敏感的超氧化物产生位点。线粒体与大鼠相比，鸽线粒体的Delta pH显著低于大鼠，但这种差异不足以解释过氧化氢生成速率较低的原因。通过线粒体黄素单核苷酸含量和蓝色天然聚丙烯酰胺凝胶电泳分析，鸽线粒体中复合物I含量低于大鼠。重新计算表明，在大鼠和鸽子中，每分子复合体I的过氧化氢产量是相同的。我们得出结论，来自长寿鸽子的线粒体显示出低过氧化氢生产速率，因为它们具有低水平的复合体I。
P>Across a range of vertebrate species, it is known that there is a negative association between maximum lifespan and mitochondrial hydrogen peroxide production. In this report, we investigate the underlying biochemical basis of the low hydrogen peroxide production rate of heart mitochondria from a long-lived species (pigeon) compared with a short-lived species with similar body mass (rat). The difference in hydrogen peroxide efflux rate was not explained by differences in either superoxide dismutase activity or hydrogen peroxide removal capacity. During succinate oxidation, the difference in hydrogen peroxide production rate between the species was localized to the Delta pH-sensitive superoxide producing site within complex I. Mitochondrial Delta pH was significantly lower in pigeon mitochondria compared with rat, but this difference in Delta pH was not great enough to explain the lower hydrogen peroxide production rate. As judged by mitochondrial flavin mononucleotide content and blue native polyacrylamide gel electrophoresis, pigeon mitochondria contained less complex I than rat mitochondria. Recalculation revealed that the rates of hydrogen peroxide production per molecule of complex I were the same in rat and pigeon. We conclude that mitochondria from the long-lived pigeon display low rates of hydrogen peroxide production because they have low levels of complex I.