Integrated Systems View on Networking by Hormones in Arabidopsis Immunity Reveals Multiple Crosstalk for Cytokinin
Integrated Systems View on Networking by Hormones in Arabidopsis Immunity Reveals Multiple Crosstalk for Cytokinin
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DOI：
10.1105/tpc.112.098335
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发表时间：
2012-05-01
期刊：
影响因子：
11.6
通讯作者：
Dandekar, Thomas
中科院分区：
文献类型：
--
作者：
Naseem, Muhammad;Philippi, Nicole;Dandekar, Thomas
植物激素信号和联合收割机维持植物体内的生理平衡。病原体通过调节植物细胞的激素平衡来增强宿主的易感性。与其他植物激素不同，细胞分裂素在植物免疫中的详细作用仍有待充分阐明。在这里，广泛的数据挖掘，包括致病因子，宿主调节蛋白，激素生物合成的酶，和信号组分，建立了一个完整的信号网络的105个节点和163条边。动态建模和系统分析确定了植物疾病网络中多种细胞分裂素介导的调控相互作用。这包括细胞分裂素和水杨酸途径之间的特定协同作用，以及植物免疫中细胞分裂素和生长素之间先前未发现的拮抗作用。预测的相互作用和激素对植物免疫力的影响在随后的实验中得到证实，假单胞菌pv番茄DC3000和拟南芥。我们的动态模拟有助于预测复杂激素疾病网络中单个组分的系统效应以及途径之间的协同作用或拮抗作用。
Phytohormones signal and combine to maintain the physiological equilibrium in the plant. Pathogens enhance host susceptibility by modulating the hormonal balance of the plant cell. Unlike other plant hormones, the detailed role of cytokinin in plant immunity remains to be fully elucidated. Here, extensive data mining, including of pathogenicity factors, host regulatory proteins, enzymes of hormone biosynthesis, and signaling components, established an integrated signaling network of 105 nodes and 163 edges. Dynamic modeling and system analysis identified multiple cytokinin-mediated regulatory interactions in plant disease networks. This includes specific synergism between cytokinin and salicylic acid pathways and previously undiscovered aspects of antagonism between cytokinin and auxin in plant immunity. Predicted interactions and hormonal effects on plant immunity are confirmed in subsequent experiments with Pseudomonas syringae pv tomato DC3000 and Arabidopsis thaliana. Our dynamic simulation is instrumental in predicting system effects of individual components in complex hormone disease networks and synergism or antagonism between pathways.