Abstract 328: Hemodynamic Comparison of Zucker Diabetic Fatty Rats to Their Lean Littermates After Asphyxial Cardiac Arrest
Abstract 328: Hemodynamic Comparison of Zucker Diabetic Fatty Rats to Their Lean Littermates After Asphyxial Cardiac Arrest
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摘要 328：窒息心脏骤停后 Zucker 糖尿病肥胖大鼠与其瘦同窝大鼠的血流动力学比较
DOI：
10.1161/circ.138.suppl_2.328
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发表时间：
2018
期刊：
影响因子：
37.8
通讯作者：
Riess ML
中科院分区：
文献类型：
--
作者：
Balzer C;Baudenbacher F;Salzman MM;Cleveland WJ;Eagle S;Riess ML
代谢综合征患者发生心脏骤停（CA）的风险较高，并且在CA后与其合并症（例如，2型糖尿病[T2 DM]）。使用Zucker糖尿病肥胖（ZDF）大鼠作为CA和心肺复苏（CPR）常见合并症的新的相关模型，我们假设与其瘦的同窝仔相比，T2 DM与自发循环（ROSC）恢复的机会较低和/或窒息CA后心脏功能的结局较差相关。监测两组大鼠（8只ZDF，7只瘦型）37±2周。将大鼠麻醉并插管;通过皮下ECG针监测心率。对股动脉和静脉插管，分别进行连续血压测量和液体和药物输送。在停止通气以开始窒息性CA之前，给予罗库溴铵。CA 8分钟后，使用FiO21.0重新开始通气，给予肾上腺素和碳酸氢钠，并开始气动胸外按压，每分钟按压200次。当达到120 mmHg的收缩压时停止胸部按压。在4小时的观察期间，密切监测生命参数，测量血气，并用超声评估射血分数（EF %）。数据为平均值± SD。统计学：非配对学生t检验（双尾），α.05。基线时，ZDF大鼠的血糖水平（504±52 vs 174±14 mg/dl）显著高于同窝瘦鼠。所有ZDF和瘦大鼠均达到ROSC，ROSC后直接和第一小时后进行的测量显示无相关差异。四小时后，ZDF和瘦大鼠之间的心率没有差异。糖尿病大鼠平均动脉血压（142±24vs. 107±19 mmHg）和射血分数（42±16%vs 20±8%）。糖尿病大鼠中ROSC率较低的假设无法得到证实。相反，ZDF大鼠显示出与EF%增加相关的显著更高的血压。本研究的进一步分析将集中于T2 DM对心脏和神经缺血-再灌注损伤的影响。
Patients with metabolic syndrome are at higher risk for cardiac arrest (CA), and also have worse neurologic outcome after CA related to their comorbidities (e.g., Type 2 Diabetes Mellitus [T2DM]). Using Zucker Diabetic Fatty (ZDF) rats as a new and relevant model with common comorbidities for CA and cardiopulmonary resuscitation (CPR), we hypothesized that T2DM is associated with a lower chance for return of spontaneous circulation (ROSC) and/or a worse outcome regarding heart function after asphyxial CA compared to their lean littermates. Two groups of rats (8 ZDF, 7 lean) were monitored for 37±2 weeks. The rats were anesthetized and intubated; heart rate was monitored by subcutaneous ECG needles. Femoral artery and vein were cannulated for continuous blood pressure measurement and delivery of fluids and medications, respectively. Before ventilation was stopped to initiate asphyxial CA, rocuronium was given. After 8 minutes of CA, ventilation was re-initiated with FiO21.0, epinephrine and sodium-bicarbonate were administered, and pneumatic chest compression were started with 200 compressions per minute. Chest compressions were stopped when a systolic blood pressure of 120 mmHg was achieved. During 4 hours of observation, vital parameters were closely monitored, blood gases were measured, and ejection fraction (EF %) was assessed with ultrasound. Data are mean ± SD. Statistics: Unpaired student’s t-test (two-tailed), α.05. At baseline, ZDF rats showed significantly higher blood glucose levels (504±52 vs 174±14 mg/dl) compared to their lean littermates. All ZDF and lean rats achieved ROSC, and measurements taken directly after ROSC and after the first hour showed no relevant differences. After four hours, there was no difference in heart rate between ZDF and lean rats. However, diabetic rats had a significantly higher mean arterial blood pressure (142±24vs. 107±19 mmHg) and ejection fraction (42±16%vs 20±8%) compared to their lean littermates. The hypothesis that ROSC-rate in diabetic rats would be lower could not be proven. Conversely, the ZDF rats showed a significantly higher blood pressure related to an increased EF%. Further analysis in this study will focus on the impact of T2DM on cardiac and neurological ischemia-reperfusion injury.