Distribution of TNF alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy
Distribution of TNF alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy
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DOI：
10.1136/bjo.80.2.168
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发表时间：
1996-02-01
影响因子：
4.1
通讯作者：
Dumonde, DC
中科院分区：
文献类型：
--
作者：
Limb, GA;Chignell, AH;Dumonde, DC
目的：研究增殖性糖尿病视网膜病变(PDR)患者眼部纤维血管膜中细胞因子肿瘤坏死因子-α(TNF-α)和血管黏附糖蛋白ICAM-1、VCAM-1、E-选择素、P-选择素和PECAM的表达情况。方法：采用免疫组织化学方法和APAAP技术，检测12例增殖性糖尿病视网膜病变(PDR)患者视网膜血管内皮细胞、膜内渗出细胞和细胞外基质中肿瘤坏死因子-α的表达。这一染色可被重组人肿瘤坏死因子α吸收的单抗所消除。同样，对9个膜的细胞和细胞外基质以及其中3个标本的内皮细胞进行ICAM-1染色。VCAM-1、E-选择素和P-选择素分别在5/12、4/12和3/12视网膜前膜的血管内皮细胞上表达。结论：肿瘤坏死因子α的广泛分布和血管内皮细胞在PDR膜上表达的黏附分子的性质提示，肿瘤坏死因子α的局部激活和血管细胞黏附分子的表达增强可能在糖尿病视网膜病变增殖期的发生发展中起重要作用。
Aims-This study investigated the presence of the cytokine tumour necrosis factor alpha(TNF alpha) and the vascular adhesion glycoproteins ICAM-1, VCAM-1, E-selectin, P-selectin, and PECAM within fibrovascular membranes of eyes with proliferative diabetic retinopathy (PDR).Methods-The presence of these molecules was determined by immunohistochemical staining using monoclonal antibodies and the APAAP technique.Results-Staining for TNF alpha was observed on the retinal vascular endothelium of five of 12 specimens, on infiltrating cells within all membranes, and on the extracellular matrix of nine specimens. This staining was abolished by absorption of the monoclonal antibody with human recombinant TNF alpha. Likewise, ICAM-1 staining was given by infiltrating cells and extracellular matrix of nine membranes and by the endothelium of three of the specimens. VCAM-1, E-selectin, and P-selectin staining was observed on the vascular endothelium of 5/12, 4/12, and 3/12 epiretinal membranes respectively. PECAM was expressed by the endothelium of 4/12 specimens, by infiltrating cells of 8/12 membranes, and also by the extracellular matrix of two of the specimens.Conclusion-The widespread distribution of TNF alpha and the nature of the adhesion molecules expressed by vascular endothelial cells in PDR membranes suggest that local activation of TNF alpha and enhanced expression of vascular cell adhesion molecules may play an important rob in the development of the proliferative phase of diabetic retinopathy.