Mining genes related to anthocyanin synthesis and regulation in different potato varieties based on comparative transcriptomics
Mining genes related to anthocyanin synthesis and regulation in different potato varieties based on comparative transcriptomics
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DOI：
10.1007/s13562-022-00816-y
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发表时间：
2023-01
影响因子：
1.9
通讯作者：
E. Qi;Xiao Jia;Helin Lv;Wei Huang;G. Wen;Zhang-Rui Li;Jian-wu Li
中科院分区：
文献类型：
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作者：
E. Qi;Xiao Jia;Helin Lv;Wei Huang;G. Wen;Zhang-Rui Li;Jian-wu Li
马铃薯块茎中的花色苷不仅赋予马铃薯鲜艳的色泽和较高的营养价值，而且具有抗氧化、降血压、降脂、增强免疫力等药理作用。然而，马铃薯块茎中参与花青素合成或调控的基因的总体表达模式仍不清楚。本研究测定了4个马铃薯品种（B、R、Y和W）的花青苷含量，并利用比较转录组学方法鉴定了不同马铃薯品种花青苷合成的调控基因。结果表明，12个测序文库共获得82.6G的筛选数据，其中82.23-87.66%的干净片段覆盖到参考基因组。在12个文库中共发现42,793个基因，其中3765个为新基因。最后，通过严格的阈值筛选，得到3638（B）、4295（R）和3594（Y）三组DEG（FDR < 0.05和|log 2光纤通道|（1）分别。GO分析表明DEGs主要富集在细胞（GO：0005623）、代谢过程（GO：0008152）、催化活性（GO：0003824）等条目中。KEGG分析表明，DEG在代谢途径（ko 01100）、苯丙氨酸代谢（ko 00360）、光合作用（ko 00195）中显著富集。此外，一些花色素苷合成基因（如CHS，bHLH和MYB）在不同品种中被高度诱导或抑制。本研究为马铃薯花色素苷形成调控基因的研究提供了理论依据，为马铃薯优质品种的选育提供了科学参考。
The anthocyanin glycosides in potato tubers not only give potato bright color and high nutritional value, but also have pharmacological effects such as antioxidant, blood pressure-lowering, lipid-lowering and immunity-boosting. However, the overall expression pattern of genes involved in anthocyanin synthesis or regulation in potato tubers remains unclear. In this study, the anthocyanin content of four potato varieties (B, R, Y and W)was measured and the regulatory genes of anthocyanin synthesis in different potato cultivars were identified by comparative transcriptomic approaches. The results showed that a total of 82.6 G of filtered data were obtained from the 12 sequencing libraries, of which 82.23–87.66% of clean fragments were covered to the reference genome. A total of 42,793 genes were found in the 12 libraries, of which 3765 were novel genes. Finally, through strict threshold screening, three groups of 3638 (B), 4295 (R) and 3594 (Y) DEGs were obtained(FDR < 0.05 and |log2FC|>1), respectively. GO analysis showed that DEGs were mainly enriched in cell (GO: 0005623), metabolic process (GO: 0008152), catalytic activity (GO: 0003824) and other entries. KEGG analysis revealed that the DEGs were significantly enriched in metabolic pathways (ko01100), phenylalanine metabolism (ko00360), photosynthesis (ko00195). In addition, some anthocyanin synthesis genes (e.g. CHS, bHLH and MYB) were highly induced or repressed in different cultivars. This study provided a theoretical basis for the study ofthe regulatory genes of anthocyanin formation in potato and a scientific reference for breeding high-quality potato cultivars.