Extended immunophenotyping reference values in a healthy pediatric population
Extended immunophenotyping reference values in a healthy pediatric population
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DOI：
10.1002/cyto.b.21728
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发表时间：
2019-05-01
影响因子：
3.4
通讯作者：
Martinez-Gallo, Monica
中科院分区：
文献类型：
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作者：
Garcia-Prat, Marina;Alvarez-Sierra, Daniel;Martinez-Gallo, Monica
背景：为了准确诊断免疫缺陷，将患者的免疫学实验室值与年龄性别匹配的对照组进行比较是至关重要的，但大多数人群缺乏正常值。目的探讨1个月~ 18岁健康儿童供体扩展淋巴细胞亚群和t细胞受体切除圈（trec）水平的适宜参考值。方法通过多参数流式细胞仪对以下亚群进行分析，获得扩展的免疫表型值：CD4+和CD8+幼稚，效应，效应记忆和中枢记忆，T辅助亚群及其激活程度，T调节细胞，最近胸腺迁移（RTE）， B淋巴细胞亚群（过渡性，幼稚，前转换记忆，开关记忆，浆母细胞，CD21low和耗散），以及单核细胞，NK细胞和树突状细胞亚群。结果获得32个淋巴细胞和单核细胞亚群的中位数、第10百分位和第90百分位，以及各年龄组儿童的trec水平。初始CD4+和CD8+ t细胞群随着年龄的增长而减少，两组之间存在显著差异，与trec计数评估的胸腺功能下降和最近的胸腺移民人群相似。细胞群的相对数量有随年龄增长而增加的趋势。在最年轻的一组和其他组之间，转换类别的B细胞群的百分比显着增加。结论本研究为解释儿童和青年人群的扩展免疫表型谱提供了必要的数据，这可能对PIDs和免疫介导疾病的诊断有价值，特别是那些与细微免疫异常相关的疾病。(c) 2018年国际临床细胞术学会
BackgroundFor the accurate diagnosis of immunodeficiencies is crucial to compare patients' immunology laboratory values with age-sex matched controls, yet there is a paucity of normal values for most populations.ObjectivesTo define appropriate reference values of extended lymphocyte subpopulations and T-cell receptor excision circle (TRECs) levels in healthy pediatric donors between 1 month and 18 years of age.MethodsExtended immunophenotyping values were obtained by analysis of multiparameter flow cytometry panels for the following subpopulations: CD4+ and CD8+ Naive, Effector, Effector Memory and Central Memory, T helper subpopulations and their degrees of activation, T Regulatory cells, Recent Thymic Emigrants (RTE), B Lymphocyte subpopulations (Transitional, Naive, Preswitch-Memory, Switch-Memory, Plasmablasts, CD21low, and Exhausted), and subpopulations for Monocytes, NK cells and Dendritic Cells.ResultsMedian values and the 10th and 90th percentiles were obtained for 32 lymphocyte and monocyte subpopulations, and for TRECs levels in each age group of children. Naive CD4+ and CD8+ T-cell populations tended to decrease with age, with significant difference between the groups, in parallel with the reduction in thymic function assessed by TRECs counts and the recent thymic emigrant population. Relative numbers of Th cell populations tended to increase with age. The percentage of class-switched B cell populations showed a significant increase between the youngest group and the others.ConclusionThis study provides essential data for interpreting extended immunophenotyping profiles in the pediatric and young adult populations, which could be of value for the diagnosis of PIDs and immune-mediated diseases, particularly those associated with subtle immunological abnormalities. (c) 2018 International Clinical Cytometry Society