Isolation of the brassinosteroid receptor genes and recharacterization of dwarf plants by silencing of SlBRI1 in tomato
Isolation of the brassinosteroid receptor genes and recharacterization of dwarf plants by silencing of SlBRI1 in tomato
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番茄中油菜素类固醇受体基因的分离和通过沉默 SlBRI1 来重新表征矮化植物
DOI：
10.1007/s10725-019-00524-z
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发表时间：
2019
影响因子：
4.2
通讯作者：
Hu Zongli
中科院分区：
文献类型：
--
作者：
Zhu Zhiguo;Liang Honglian;Chen Guoping;Tang Boyan;Tian Shibing;Hu Zongli
类甾醇（BR）是一种甾体激素，在植物生长发育过程中起重要作用，包括细胞分裂和伸长，以及植物抗病性等。BR受体BRASSINOSTEROPHYSITIVE 1（BRI 1）是一种多结构域跨膜受体样激酶，在BR识别和信号传导中发挥作用。在这项研究中，两个假定的番茄BR受体基因被鉴定，SlBRI 1-like 1（SlBRL 1）和SlBRI 1-like 2（SlBRL 2）。与已知受体基因SlBRI 1/CURL 3一起，这三种BR受体在BR识别和激酶激活功能上具有保守的序列和构象。其中，SlBR 1在多种组织中高表达，而SlBR 1在茎中转录丰度更高，表明它们在番茄生长发育中可能具有多样性的作用。与SlBRI 1的表达特异性相对应，SlBRI 1-RNAi植株表现出典型的株高降低、叶片卷曲、绿色、节间缩短的表型。同时，SlBRI 1的下调可能通过一条不依赖于SlSTYLE2.1的途径使花的花柱短于野生型。此外，BR生物合成基因SlCPD、SlBRI 1、SlDET 2的表达上调和BR失活基因SlCYP 734 A7的表达下调揭示了SlBRI 1在BR途径介导的植物构型和反馈调节中的重要作用。
Brassinosteroid (BR) is a kind of steroid hormone that is essential for the regulation of multiple processes of plant growth and development, including cell division and elongation, as well as plant resistance to diseases. The BR receptor BRASSINOSTEROID INSENSITIVE1 (BRI1), a multidomain transmembrane receptor-like kinase, roles in BR recognition and signaling. In this study, two putative tomato BR receptor genes were identified,SlBRI1-like 1(SlBRL1) andSlBRI1-like 2(SlBRL2). Together with the known receptor geneSlBRI1/CURL3, these three BR receptors had conserved sequence and conformation for their function in BR recognition and kinase activation. Among them, theSlBRI1was highly expressed in multiple tissues whileSlBRLshad a more abundant transcription in the stem, suggesting that they may have a diverse role in tomato growth and development. Corresponding to the expression specificity ofSlBRI1, the SlBRI1-RNAi plants exhibited the typical phenotype of reduced plant height, curly dark green leaves and shortened internodes. Meanwhile, the down-regulation ofSlBRI1gave the flower a shorter style than that of wild-type possibly via aSlSTYLE2.1-independent pathway. Additionally, the elevated expression of BR biosynthesis genesSlCPD,SlDWF,SlDET2and repressed expression of BR inactivation geneSlCYP734A7revealed the critical role ofSlBRI1in BR pathway-mediated plant architecture and the feedback regulation.