Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain.
Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain.
复制标题
大鼠脑S-100蛋白的分子克隆和cDNA到mRNA的完整核苷酸序列。
DOI：
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发表时间：
1984
影响因子：
14.9
通讯作者：
Yasuo Takahashi
中科院分区：
文献类型：
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作者：
Ryozo Kuwano;Hiroshi Usui;T. Maeda;Toshikazu Fukui;Nobuko Yamanari;Eiko Ohtsuka;Morio Ikehara;Yasuo Takahashi
从重组cDNA克隆中测定了大鼠脑S-100蛋白β亚基mRNA的全核苷酸序列。该序列全长1488 bp，包括276 bp的完整编码区、120 bp的5 ′端非编码区和1092 bp的3 ′端非编码区，其中3 ′端非编码区含有两个多聚腺苷酸化信号。此外，还发现了poly（A）尾，其核苷酸序列推导的氨基酸序列与牛S-100 β亚基的氨基酸序列除4个氨基酸残基显示种属差异外，其余氨基酸序列均与牛S-100 β亚基的氨基酸序列同源。从进化意义的角度，S-100的核苷酸序列与大鼠肠钙结合蛋白（ICaBP）和钙调素（CaM）的核苷酸序列之间的同源性进行了检查。用标记的cDNA对发育中的大鼠脑进行了poly（A）RNA的斑点杂交，结果表明，S-100 mRNA在出生后10-20天迅速增加。还描述了C-6胶质瘤细胞中S-100 mRNA的存在。
The complete nucleotide sequence of mRNA for beta-subunit of rat brain S-100 protein was determined from recombinant cDNA clones. The sequence was composed of 1488 bp which included the 276 bp of the complete coding region, the 120 bp of the 5'-noncoding region and the 1092 bp of the 3'-noncoding region containing two polyadenylation signals. In addition, the poly(A) tail was also found. The amino acid sequence deduced from the nucleotide sequence was homologous to the amino acid sequence of bovine S-100 beta subunit except 4 residues showing species differences. From the viewpoint of evolutionary implications, the homology between the nucleotide sequence of S-100 and those of rat intestinal Ca-binding protein (ICaBP) and calmodulin (CaM) was examined. A dot-blot hybridization of poly(A) RNA from the developing rat brains using a labeled cDNA showed a rapid increase in S-100 mRNA at 10-20 postnatal days. The presence of S-100 mRNA in C-6 glioma cells is also described.