Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor
Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor
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DOI：
10.1104/pp.123.1.93
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发表时间：
2000-05-01
期刊：
影响因子：
7.4
通讯作者：
Yoshida, S
中科院分区：
文献类型：
--
作者：
Asami, T;Min, YK;Yoshida, S
对油菜素类固醇(BR)生物合成抑制剂进行了筛选，以寻找在拟南芥中诱导矮化的化学物质，这些突变体类似于BR生物合成突变体，可以被BR拯救。通过本次筛选实验，筛选出药效最强的化合物为油菜素。在暗培养拟南芥中，用油菜素内酯处理后，油菜素内酯诱导的形态变化几乎恢复到野生型的水平。油菜素的结构类似于赤霉素类生物合成抑制剂帕克曲唑。然而，在对水芹的测定中，油菜唑处理的植株在添加赤霉素后没有恢复，但在添加油菜素内酯后表现出良好的恢复，这些数据表明油菜唑是一种特异的BR生物合成抑制剂。用油菜素内酯处理的水芹也表现出矮化，叶片形态发生变化，包括拟南芥BR缺失突变体典型的向下卷曲和深绿色，这种矮化可以被10 NM的油菜素内酯逆转。这一结果表明，BRs对植物的生长是必需的，油菜唑可以用来阐明BRs在植物中的功能，作为对BR缺乏突变体的补充。通过向光照下生长的水芹饲喂BR生物合成中间体，研究了油菜素的作用部位。
Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by BR. Through this screening experiment, the compound brassinazole was selected as the most potent chemical. In dark-grown Arabidopsis, brassinazole-induced morphological changes were nearly restored to those of wild type by treatment with brassinolide. The structure of brassinazole is similar to pacrobutrazol, a gibberellin biosynthesis inhibitor. However, in assays with cress (Lepidium sativum) plants, brassinazole-treated plants did not show recovery after the addition of gibberellin but showed good recovery after the addition of brassinolide, These data demonstrate that brassinazole is a specific BR biosynthesis inhibitor. Brassinazole-treated cress also showed dwarfism, with altered leaf morphology, including the downward curling and dark green color typical of Arabidopsis BR-deficient mutants, and this dwarfism was reversed by the application of 10 nM brassinolide. This result suggests that BRs are essential for plant growth, and that brassinazole can be used to clarify the function of BRs in plants as a complement to BR-deficient mutants. The brassinazole action site was also investigated by feeding BR biosynthesis intermediates to cress grown in the light.