Influenza M2 virus-like particles confer a broader range of cross protection to the strain-specific pre-existing immunity.
Influenza M2 virus-like particles confer a broader range of cross protection to the strain-specific pre-existing immunity.
复制标题
DOI：
10.1016/j.vaccine.2014.08.030
复制
发表时间：
2014-10-07
期刊：
影响因子：
5.5
通讯作者：
Kang, Sang-Moo
中科院分区：
文献类型：
--
作者：
Kim, Min-Chul;Lee, Yu-Na;Hwang, Hye Suk;Lee, Young-Tae;Ko, Eun-Ju;Jung, Yu-Jin;Cho, Min Kyoung;Kim, Yu-Jin;Lee, Jong Seok;Ha, Suk-Hoon;Kang, Sang-Moo
每年接种一次疫苗的人体免疫力不能有效保护人体免受抗原性不同的菌株的侵害。作为在存在预先存在的毒株特异性免疫的情况下改善交叉保护的方法，我们在先前接种疫苗的小鼠中在随后用保守抗原性靶向流感M2胞外域（M2 e）病毒样颗粒疫苗（M2 e5 x VLP）免疫后的较早时间研究了异源和异亚型保护的功效。用H1N1裂解疫苗免疫小鼠诱导了针对同源流感病毒的病毒特异性抗体，但没有提供异亚型血凝抑制抗体应答和交叉保护。然而，随后的M2 e5 x VLP免疫诱导M2 e特异性抗体应答以及全身和粘膜部位的干扰素-γ（IFN-γ）产生细胞。在用H3 N2或H5 N1亚型流感病毒致死攻击后，随后用M2 e5 x VLP免疫的小鼠被很好地保护免受异亚型流感病毒的侵害。这些结果提供了证据表明，非季节性免疫接种M2 e5 x VLP，通用疫苗的实验候选人，是一种有前途的方法，扩大交叉保护，即使在株特异性免疫的存在。
Immunity in humans with annual vaccination does not provide effective protection against antigenically distinct strains. As an approach to improve cross-protection in the presence of pre-existing strain-specific immunity, we investigated the efficacy of heterologous and heterosubtypic protection in previously vaccinated mice at earlier times after subsequent immunization with conserved-antigenic target influenza M2 ectodomain (M2e) virus-like particle vaccine (M2e5x VLP). Immunization of mice with H1N1 split vaccine induced virus specific antibodies to homologous influenza virus but did not provide heterosubtypic hemagglutination inhibiting antibody responses and cross-protection. However, subsequent M2e5x VLP immunization induced M2e specific antibody response as well as interferon-γ (IFN-γ) producing cells in systemic and mucosal sites. Upon lethal challenge with H3N2 or H5N1 subtype influenza viruses, subsequently immunized mice with M2e5x VLP were well protected against heterosubtypic influenza viruses. These results provide evidence that non-seasonal immunization with M2e5x VLP, an experimental candidate for universal vaccine, is a promising approach for broadening the cross-protection even in the presence of strain-specific immunity.
登录
查看更多内容
影响因子：
5.4
作者：
Quan, Fu-Shi;Compans, Richard W.;Kang, Sang-Moo
通讯作者：
Kang, Sang-Moo
影响因子：
5.4
作者：
Jayasekera, Jerome P.;Moseman, E. Ashley;Carroll, Michael C.
通讯作者：
Carroll, Michael C.
影响因子：
5.4
作者：
Grantham, Michael L.;Stewart, Shaun M.;Pekosz, Andrew
通讯作者：
Pekosz, Andrew
DOI：
10.1073/pnas.1132114100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Plotkin, JB;Dushoff, J
通讯作者：
Dushoff, J
影响因子：
12.4
作者：
Kim, Min-Chul;Song, Jae-Min;Kang, Sang-Moo
通讯作者：
Kang, Sang-Moo