Liposomes from novel photolabile phospholipids: Light-induced unloading of small molecules as monitored by PFG NMR
Liposomes from novel photolabile phospholipids: Light-induced unloading of small molecules as monitored by PFG NMR
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DOI：
10.1021/ja016874i
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发表时间：
2002-05-22
影响因子：
15
通讯作者：
Kutateladze, AG
中科院分区：
文献类型：
--
作者：
Wan, YQ;Angleson, JK;Kutateladze, AG
发展了一种基于分子二噻烷的合成新型光不稳定磷脂的方法。这些脂质与蛋-POPC和胆固醇一起用于制剂中以制备光敏脂质体。在PBS缓冲液（中压汞灯，Pyrex过滤器，λ > 300 nm）中照射这种脂质体显著增加双层渗透性，并将截留的小有机分子的释放加速一个数量级。一个简单的分析，基于1H或19 F PFG NMR测量的扩散系数，开发监测光诱导卸载的探针分子。
A molecular dithiane-based approach to synthesis of novel photolabile phospholipids is developed. These lipids are used in formulations with egg-POPC and cholesterol to prepare light-sensitive liposomes. Irradiation of such liposomes in PBS buffer (medium pressure mercury lamp, Pyrex filter, λ > 300 nm) significantly increases the bilayer permeability and accelerates the release of entrapped small organic molecules by an order of magnitude. A simple assay, based on1H or19F PFG NMR measurements of diffusion coefficients, is develped to monitor light-induced unloading of the probe molecules.