Comparison between waste frying oil and paraffin as carbon source in the production of biodemulsifier by Dietzia sp. S-JS-1.
Comparison between waste frying oil and paraffin as carbon source in the production of biodemulsifier by Dietzia sp. S-JS-1.
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DOI：
10.1016/j.biortech.2009.07.006
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发表时间：
2009-12
影响因子：
11.4
通讯作者：
Jia Liu;Xiang-feng Huang;Li-jun Lu;Jing-cheng Xu;Y. Wen;Dianhai Yang;Qi Zhou
中科院分区：
文献类型：
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作者：
Jia Liu;Xiang-feng Huang;Li-jun Lu;Jing-cheng Xu;Y. Wen;Dianhai Yang;Qi Zhou
为了降低生产成本，利用废弃煎炸油为原料，从石油污染土壤中分离得到一株Dietzia sp. S-JS-1菌株，并将其用于生物合成破乳剂。经过7 d的培养，最适宜的废弃煎炸油（WFO Ⅱ）培养物的生物量浓度达到3.78g/L，是石蜡培养物的2.4倍。在相同的碳源体积比和培养条件下，WFO II培养物的破乳效果优于石蜡培养物。在5 h内，对W/O型乳状液的油分离率达到88.3%，对O/W型乳状液的水分离率达到76.4%。借助薄层色谱（TLC）和傅里叶变换红外（FTIR）光谱，确定了石蜡和WFO II产生的生物破乳剂为脂肽同系物的混合物。这些同系物分布的细微变化和WFO II培养物的高生物量浓度可以解释上述良好的破乳性能。
In order to lower the production cost, waste frying oils were used in the biosynthesis of demulsifier by Dietzia sp. S-JS-1, which was isolated from petroleum contaminated soil. After 7days of cultivation, the biomass concentration of the most suitable waste frying oil (WFO II) culture reached 3.78g/L, which was 2.4times the concentration of paraffin culture. The biodemulsifier produced with WFO II culture broke the emulsions more efficiently than that produced with paraffin culture, given the same volume ratio of carbon source in the culture medium and the same cultivation conditions. It achieved 88.3% of oil separation ratio in W/O emulsion and 76.4% of water separation ratio in O/W emulsion within 5h. With the aid of thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectrometry, biodemulsifiers produced from both paraffin and WFO II were identified as a mixture of lipopeptide homologues. The subtle variation in the distribution of these homologues and high biomass concentration of WFO II cultures may account for the afore-mentioned good demulsification performance.