ASSOCIATION OF FACTOR-XI AND HIGH MOLECULAR-WEIGHT KININOGEN IN HUMAN-PLASMA
ASSOCIATION OF FACTOR-XI AND HIGH MOLECULAR-WEIGHT KININOGEN IN HUMAN-PLASMA
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DOI：
10.1172/jci108898
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发表时间：
1977-01-01
影响因子：
15.9
通讯作者：
KAPLAN, AP
中科院分区：
文献类型：
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作者：
THOMPSON, RE;MANDLE, R;KAPLAN, AP
通过Sephadex G-200上的凝胶过滤评估，在MW 380，000的正常人血浆中，因子XI和高MW激肽原相关。高MW激肽原缺陷血浆中因子XI的MW为175，000，与纯化因子XI获得的值相同。当用纯化的高MW激肽原重构高MW激肽原缺陷血浆时，发现所有因子XI的MW为380，000。在因子XI和高度纯化的高MW激肽原孵育后也可证明复合物形成。该复合物不同于前激肽释放酶-高MW激肽原复合物;高MW激肽原与因子XI或前激肽释放酶形成双分子复合物，但不形成包括因子XI和前激肽释放酶两者的三分子复合物。既不Hageman因子，也不纤溶酶原与高分子量激肽原;结合高分子量激肽原似乎是一个特定的属性的Hageman因子基板。
Factor XI and high MW kininogen were associated in normal human plasma at MW 380,000 as assessed by gel filtration on Sephadex G-200. The MW of Factor XI in high MW kininogen-deficient plasma was 175,000, the same value obtained for purified Factor XI. When high MW kininogen-deficient plasma was reconstituted with purified high MW kininogen, all of the Factor XI was then found at MW 380,000. Complex formation was also demonstrable upon incubation of Factor XI and highly purified high MW kininogen. This complex was distinct from the prekallikrein-high MW kininogen complex; high MW kininogen forms bimolecular complexes with either Factor XI or prekallikrein but does not form a trimolecular complex that includes both Factor XI and prekallikrein. Neither Hageman factor nor plasminogen were associated with high MW kininogen; binding to high MW kininogen appeared to be a specific property of the Hageman factor substrates.