The polyamine spermine protects against high salt stress in Arabidopsis thaliana
The polyamine spermine protects against high salt stress in Arabidopsis thaliana
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DOI：
10.1016/j.febslet.2006.10.078
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发表时间：
2006-12-22
期刊：
影响因子：
3.5
通讯作者：
Kusano, Tomonobu
中科院分区：
文献类型：
--
作者：
Yamaguchi, Koji;Takahashi, Yoshihiro;Kusano, Tomonobu
众所周知，非生物条件如离子浓度、温度和湿度的变化导致植物中多胺含量的调节。然而，很少有人知道这些多胺在非生物胁迫反应中发挥的相关部分。在这里，我们解决了一个特定的作用精胺在高盐胁迫下使用拟南芥双敲除突变体植物（acl 5lsture rs），不能产生精胺。突变体对高盐胁迫的敏感性高于野生型。这种表型被治愈的外源性精胺，但不是由其他多胺腐胺和亚精胺，这表明精胺缺乏症和氯化钠过敏症之间有很强的联系。该突变体也是高水平的KCl过敏，但不MgCl 2或高维生素C。突变体的NaCl-超敏反应受到Ca 2+通道阻滞剂治疗的影响。此外，该突变体在Ca 2+耗尽的Murashige-Skoog琼脂培养基上表现出较差的生长。这些数据表明，精胺的情况下导致突变体植物中的Ca 2+稳态失衡。基于所获得的数据，我们提出了一个模型，精胺在高盐胁迫反应中的作用。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
It is well known that changes in abiotic conditions such as the concentration of ions, temperature and humidity lead to modulation of polyamine contents in plants. However, little is known about the relevant parts these polyamines play in abiotic stress responses. Here we addressed a specific role of spermine during high salt stress using an Arabidopsis double knockoutmutant plant (acl5lspnrs) which cannot produce spermine. The mutant showed higher sensitivity to high salt than wild type plants. This phenotype was cured by exogenous spermine but not by the other polyamines putrescine and spermidine, suggesting a strong link between spermine-deficiency and NaCl-hypersensitivity. The mutant was also hypersensitive to high levels of KCl but not to MgCl2 or to high osmoticum. NaCl-hypersensitivity of the mutant was compromised by treatment with Ca2+ channel blockers. Moreover, the mutant showed poor growth on Ca2+-depleted Murashige-Skoog agar media. The data suggest that the absence of spermine causes an imbalance in Ca2+ homeostasis in the mutant plant. Based on the data obtained, we propose a model for a role of spermine in high salt stress responses. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.