Oxalic Acid Mitigates Cadmium Toxicity in Cicer arietinum L. Germinating Seeds by Maintaining the Cellular Redox Homeostasis
Oxalic Acid Mitigates Cadmium Toxicity in Cicer arietinum L. Germinating Seeds by Maintaining the Cellular Redox Homeostasis
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DOI：
10.1007/s00344-021-10334-1
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发表时间：
2021-02
影响因子：
4.8
通讯作者：
Lamia Sakouhi;O. Kharbech;Marouane Ben Massoud;S. Munemasa;Y. Murata;A. Chaoui
中科院分区：
文献类型：
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作者：
Lamia Sakouhi;O. Kharbech;Marouane Ben Massoud;S. Munemasa;Y. Murata;A. Chaoui
研究了草酸（OA）对镉（Cd）毒害的保护作用。鹰嘴豆（Cicer arietinumL.）将种子暴露于200 μM Cd胁迫6天或在3天Cd胁迫后用200 μM Cd + 100 μM OA共处理3天。OA的施用促进了镉处理幼苗的根和芽的生长。这一效应是通过限制Cd在植物组织中的积累来实现的。此外，OA处理的幼苗中丙二醛（MDA）和羰基含量下降，表明OA逆转了镉诱导的氧化应激及其对细胞膜完整性的不利影响。这些结果进一步证实了减少2-和1.7倍的过氧化氢（H2 O2）的积累在根和芽，分别相比，镉挑战的幼苗。此外，OA主要通过恢复谷胱甘肽库来纠正镉引起的谷胱甘肽氧化还原状态的失衡。这一成就似乎是谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）活性调节的结果。同样，OA抵消了镉对烟酰胺氧化还原状态的不利影响，反映了氧化[NAD（P）]和还原[NAD（P）H]形式之间的平衡的恢复。综上所述，我们的研究结果表明，外源供应OA发芽种子可以是一个很有前途的替代，以提高植物对重金属胁迫的耐受性。
The present study aimed to investigate the potential protective role of oxalic acid (OA) against Cadmium (Cd) toxicity. Chickpea (Cicer arietinumL.) seeds were exposed to 200 µM Cd stress for 6 days or to co-treatment with 200 µM Cd + 100 µM OA for 3 days following to 3-day Cd stress. The application of OA ameliorated the growth of both roots and shoots of Cd-treated seedlings. This effect was mediated by the restriction of Cd accumulation in plant tissues. Besides, malondialdehyde (MDA) and carbonyl group contents decreased in OA-treated seedlings, suggesting that OA reversed the Cd-induced oxidative stress and its detrimental effect on cell membrane integrity. These results were further confirmed by the reduction by 2- and 1.7-fold of hydrogen peroxide (H2O2) accrual in roots and shoots, respectively, when compared to Cd-challenged seedlings. Moreover, OA corrected the Cd-imposed imbalance of the glutathione redox state, mainly via the restoration of the glutathione pool. This achievement seems to be the result of the modulation of glutathione peroxidase (GPX) and glutathione reductase (GR) activities. Likewise, OA counteracted the adverse effect of Cd on nicotinamides redox state reflected by the restoration of the balance between oxidized [NAD(P)] and reduced [NAD(P)H] forms. Taken together, our results suggest that the exogenous supply of OA to germinating seeds can be a promising alternative to improve plant tolerance to heavy metal stress.