In vivo monitoring of arterially transplanted bone marrow mononuclear cells in a rat transient focal brain ischemia model using magnetic resonance imaging
In vivo monitoring of arterially transplanted bone marrow mononuclear cells in a rat transient focal brain ischemia model using magnetic resonance imaging
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DOI：
10.1179/1743132813y.0000000171
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发表时间：
2013-07-01
影响因子：
1.9
通讯作者：
Katayama, Yasuo
中科院分区：
文献类型：
--
作者：
Kamiya, Nobuo;Ueda, Masayuki;Katayama, Yasuo
目的：骨髓单个核细胞(BMMCs)动脉内移植能有效改善神经元功能，缩小梗塞范围。用高场磁共振成像(MRI)监测超顺磁性氧化铁(SPIO)标记的BMMCs移植后7d的存活情况。方法：雄性SD大鼠采用线栓法造成90min的局灶性脑缺血模型，再灌注后立即经同侧颈动脉移植16107个BMMCs或载体剂。自体骨髓细胞在缺血前用SPIO电穿孔标记。分别于再灌流后1小时、24小时、3天、7天进行MRI检查。分别在T2加权像和T2加权像上测量梗死灶的总体积和负点体积。结果：BMMC移植1h后，证实BMMC在缺血侧广泛分布为负点。以大脑半球体积归一化的负点体积迅速减少，在移植后第7天很少见到。在再灌注后24小时和7天，移植组的脑梗塞体积明显小于赋形剂组。讨论：本研究建立了大鼠短暂性局灶性脑缺血模型动脉内移植SPIO标记的BMMCs的在体监测。缺血急性期BMMCs在缺血灶中的积聚可能是限制梗塞范围的条件之一。
Objectives: Intra-arterial transplantation of bone marrow mononuclear cells (BMMCs) effectively improves neuronal function and limits the infarct size. We monitored the fate of BMMCs labeled with super paramagnetic iron oxide (SPIO) until 7 days after the transplantation using high-field magnetic resonance imaging (MRI).Methods: Male Sprague-Dawley rats were subjected to 90-minute focal ischemia using the intraluminal suture technique followed by transplantation of 1610 7 BMMCs or vehicle only via the ipsilateral carotid artery immediately after reperfusion. Autologous BMMCs were labeled with SPIO by electroporation prior to ischemia. MRI studies were performed at 1 hour, 24 hours, 3 days, and 7 days after reperfusion on each rat. The total infarct volume and the volume of negative dots were measured on T2-weighted images and T2*-weighted images, respectively.Results: One hour after BMMC transplantation, we confirmed wide spread distribution of BMMCs in the ischemic hemisphere as a negative dot. The volume of negative dot normalized by hemispheric volume decreased rapidly and was seldom seen at the seventh day after transplantation. The infarct volume was significantly smaller in the transplanted group than the vehicle group at 24 hours and 7 days after reperfusion.Discussion: The present study established in vivo monitoring of intra-arterial transplanted SPIO-labeled BMMCs immediately after reperfusion using MRI of a rat transient focal ischemia model. The accumulation of BMMCs in ischemic lesion at the acute stage of ischemia can be part of the conditions to limit the infarct size.