Ulcerative Colitis: Novel Epithelial Insights Provided by Single Cell RNA Sequencing.
Ulcerative Colitis: Novel Epithelial Insights Provided by Single Cell RNA Sequencing.
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DOI：
10.3389/fmed.2022.868508
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发表时间：
2022
影响因子：
3.9
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中科院分区：
文献类型：
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溃疡性结肠炎(UC)是一种慢性肠道炎症性疾病，其确切病因尚不清楚。遗传因素和环境因素共同参与了UC的发生发展。最近，单细胞RNA测序(scRNA-seq)技术揭示了与UC发病有关的细胞亚群，并为连接基因组与病理的途径提供了新的见解。这篇综述描述了布罗德研究所和牛津大学的两项主要研究中的关键scRNA-seq发现，这两项研究调查了UC中上皮细胞的转录格局。我们集中于五个主要发现：(1)BEST4+细胞的鉴定，(2)结肠微折叠(M)细胞，(3)杯状细胞转录本的详细比较，(4)克隆细胞和(5)健康和疾病中的干细胞。在分析这两项研究时，我们找出了方法、结果和结论上的共同点和不同点，提供了可能的解释，并验证了几个细胞簇标记。在将结果系统化的过程中，我们希望提供一个框架，让广大的胃肠道科学界和胃肠道临床医生可以用来复制或证实RNA-SEQ提供的广泛的新发现。
Ulcerative Colitis (UC) is a chronic inflammatory disease of the intestinal tract for which a definitive etiology is yet unknown. Both genetic and environmental factors have been implicated in the development of UC. Recently, single cell RNA sequencing (scRNA-seq) technology revealed cell subpopulations contributing to the pathogenesis of UC and brought new insight into the pathways that connect genome to pathology. This review describes key scRNA-seq findings in two major studies by Broad Institute and University of Oxford, investigating the transcriptomic landscape of epithelial cells in UC. We focus on five major findings: (1) the identification of BEST4 + cells, (2) colonic microfold (M) cells, (3) detailed comparison of the transcriptomes of goblet cells, and (4) colonocytes and (5) stem cells in health and disease. In analyzing the two studies, we identify the commonalities and differences in methodologies, results, and conclusions, offering possible explanations, and validated several cell cluster markers. In systematizing the results, we hope to offer a framework that the broad scientific GI community and GI clinicians can use to replicate or corroborate the extensive new findings that RNA-seq offers.