OPTICAL RECORDING OF NEURONAL-ACTIVITY IN AN INVERTEBRATE CENTRAL NERVOUS-SYSTEM - SIMULTANEOUS MONITORING OF SEVERAL NEURONS

OPTICAL RECORDING OF NEURONAL-ACTIVITY IN AN INVERTEBRATE CENTRAL NERVOUS-SYSTEM - SIMULTANEOUS MONITORING OF SEVERAL NEURONS
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DOI:
10.1152/jn.1977.40.6.1281
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发表时间:
1977-01-01
影响因子:
2.5
通讯作者:
ROSS, WN
ROSS, WN
中科院分区:
医学3区
文献类型:
--
作者:
SALZBERG, BM;GRINVALD, A;ROSS, WN

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吸收的变化,发生在染色的巨大轴突在动作电位被用来监测动作电位和突触电位的藤壶,藤壶的食管上神经节的细胞体。将神经节与部花青-恶唑酮染料孵育15分钟,该染料在鱿鱼轴突实验中给出了相对较大的信号。可检测到的最小突触电位为. apprx。5 mV。可以同时和单独监测14个神经元的动作电位活动。
Changes in absorption that occur in stained giant axons during action potentials were used to monitor action potentials and synaptic potentials in cell bodies of the supraesophageal ganglion of the barnacle, Balanus nubilus. The ganglia were incubated for 15 min with a merocyanine-oxazolone dye that had given relatively large signals in experiments on squid axons. The smallest synaptic potential that could be detected was .apprx. 5 mV. It was possible to monitor action potential activity in 14 neurons simultaneously and individually.