Self-Recognition in Social Amoebae Is Mediated by Allelic Pairs of Tiger Genes
Self-Recognition in Social Amoebae Is Mediated by Allelic Pairs of Tiger Genes
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DOI：
10.1126/science.1203903
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发表时间：
2011-07-22
期刊：
影响因子：
56.9
通讯作者：
Shaulsky, Gad
中科院分区：
文献类型：
--
作者：
Hirose, Shigenori;Benabentos, Rocio;Shaulsky, Gad
社会变形虫的自由生活细胞可以聚集和发育成多细胞子实体，其中许多细胞无私地死亡，因为它们成为支持幸存孢子的柄细胞。网柄网柄菌细胞表现出亲缘识别--一种潜在的防御作弊者的方法，作弊者会产生孢子，但不会对柄有贡献。亲缘关系取决于菌株间的亲缘关系，而多态基因tgrB1和tgrC1是这一机制的潜在组成部分。在这里，我们展示了这些基因在亲属歧视中的直接作用。我们证明，一对匹配的tgrB1和tgrC1等位基因是有吸引力的自我识别的必要条件和充分条件，这是由细胞与细胞之间的不同黏附所介导的。我们认为TgrB1和TgrC1蛋白通过直接结合来介导这种黏附。这个系统是真核生物自我识别的一个基因上容易处理的古老模型。
Free-living cells of the social amoebae Dictyostelium discoideum can aggregate and develop into multicellular fruiting bodies in which many die altruistically as they become stalk cells that support the surviving spores. Dictyostelium cells exhibit kin discrimination-a potential defense against cheaters, which sporulate without contributing to the stalk. Kin discrimination depends on strain relatedness, and the polymorphic genes tgrB1 and tgrC1 are potential components of that mechanism. Here, we demonstrate a direct role for these genes in kin discrimination. We show that a matching pair of tgrB1 and tgrC1 alleles is necessary and sufficient for attractive self-recognition, which is mediated by differential cell-cell adhesion. We propose that TgrB1 and TgrC1 proteins mediate this adhesion through direct binding. This system is a genetically tractable ancient model of eukaryotic self-recognition.